Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00007041-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007041-M01, RRID:AB_2303223
- Product name
- Mouse Monoclonal HIC5/TGFB1I1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TGFB1I1 - transforming growth factor beta 1 induced transcript 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - Analysis of TGFB1I1 over-expressed 293 cell line, cotransfected with TGFB1I1 Validated Chimera RNAi ( Cat # H00007041-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 (Cat # H00007041-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - Analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 monoclonal antibody (M01), clone 4B2-D8.Lane 1: TGFB1I1 transfected lysate(47.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - TGFB1I1 monoclonal antibody (M01), clone 4B2-D8 Analysis of TGFB1I1 expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: HIC5/TGFB1I1 Antibody (4B2-D8) [H00007041-M01] - Detection limit for recombinant GST tagged TGFB1I1 is approximately 0.03ng/ml as a capture antibody.
