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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-28839 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Hic-5 tansfected 293T cell. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references GPR75 receptor mediates 20-HETE-signaling and metastatic features of androgen-insensitive prostate cancer cells.
Basement membrane ligands initiate distinct signalling networks to direct cell shape.
Cárdenas S, Colombero C, Panelo L, Dakarapu R, Falck JR, Costas MA, Nowicki S
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Feb;1865(2):158573
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Feb;1865(2):158573
Basement membrane ligands initiate distinct signalling networks to direct cell shape.
Randles MJ, Lausecker F, Humphries JD, Byron A, Clark SJ, Miner JH, Zent R, Humphries MJ, Lennon R
Matrix biology : journal of the International Society for Matrix Biology 2020 Aug;90:61-78
Matrix biology : journal of the International Society for Matrix Biology 2020 Aug;90:61-78
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 polyclonal antibody. Lane A: Non-transfected lysate. Lane B: TGFB1I1 transfected lysate. 12% SDS PAGE. HIC5 Polyclonal Antibody (Product # PA5-28839) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HIC5 in paraformaldehyde-fixed U2OS cells using a HIC5 polyclonal antibody (Product # PA5-28839) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
