600-401-A91

antibody from Invitrogen Antibodies

Targeting: CENPU CENP-50, CENP-U, KLIP1, MLF1IP, PBIP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-A91

Provider

Invitrogen Antibodies

Product name

MLF1IP / PBIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Concentration

1.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot using Rocklands affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of rabbit anti-MLF1IP-PBIP1 antibody. Tissue: skin testis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1IP-PBIP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1IP-PBIP1 as precipitated red signal with hematoxylin purple nuclear counterstain.