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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- 10295-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIAO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- 10295-1-AP detected 48-55 kDa band because of phosphorylation. 10295-1-AP does well in IHC and IF detection.
- Concentration
- 0.27 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP ( CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP ( CIAO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 10295-1-AP (CIAO1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 10295-1-AP ( CIAO1 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 10295-1-AP ( CIAO1 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 10295-1-AP ( CIAO1 Antibody) at dilution of 1:200 (under 40x lens).
