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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C142861 - Provider product page
- Provider
- LSBio
- Product name
- Capicua / CIC Antibody (aa1500-1608, DY650) LS-C142861
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Conjugate
- Red dye
- Storage
- Store at 4°C. Do not freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human CIC/Capicua by Western Blot and Immunoprecipitation. Samples: 1 mg for IP, 20% of IP loaded and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- CIC antibody used for WB at 1 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate. CIC was also immunoprecipitated by rabbit anti-CIC antibody which recognizes an upstream epitope. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of CIC from HeLa cell lysates using antibody Capicua / CIC Antibody. Lane 2 shows extracts immunoprecipitated with an IgG control antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of CIC from HeLa cell lysates using antibody Capicua / CIC Antibody. Lane 2 shows extracts immunoprecipitated with an IgG control antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of CIC from HeLa cell lysates using antibody Capicua / CIC Antibody. Lane 2 shows extracts immunoprecipitated with an IgG control antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-CIC antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-CIC antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
