Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-83721 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PKRKMRRRSS CSSEPNTPKS AKCEGDIFTF DRTGTEAEDV LGELEYDKVP YSSLRRTLDQ RRALVMQLFQ DHGFFPSAQA TAAFQARYAD IFPSKVCLQL KIREVRQKIM QA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIC by a CIC polyclonal antibody (Product # PA5-83721). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line A-549.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIC by a CIC polyclonal antibody (Product # PA5-83721). Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CIC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CIC in U-2 OS cells using a CIC polyclonal antibody (Product # PA5-83721). The analysis shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CIC in human testis and liver tissues using a CIC polyclonal antibody (Product # PA5-83721). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CIC in human cerebellum using a CIC polyclonal antibody (Product # PA5-83721). The analysis shows strong nuclear immunoreactivity in Purkinje cells, as well as positivity in neurons in granular and molecular layers.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CIC in human testis using a CIC polyclonal antibody (Product # PA5-83721). The analysis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CIC in human endometrium using a CIC polyclonal antibody (Product # PA5-83721). The analysis shows moderate to strong nuclear positivity in glandular ans stromal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CIC in human liver using a CIC polyclonal antibody (Product # PA5-83721). The analysis shows very weak nuclear positivity in hepatocytes.