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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-24603 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WWTR1 Monoclonal Antibody (CL0370)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MNPKPSSWRK KILPESFFKE PDSGSHSRQS STDSSGGHPG PRLAGGAQHV RSHSSPASLQ LGTGAGAAGS PAQQHAHLRQ QSYDVTDELP LPPGWEMTFT ATGQRYFLNH IEKITTWQDP RKAMNQPLNH MNLHPAVSST Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0370
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG. Samples were probed using a WWTR1 Monoclonal Antibody (Product # MA5-24603).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-WWTR1 Monoclonal Antibody (CL0370) (Product # MA5-24603) and a 55 kDa band corresponding to WWTR1 was observed across cell lines tested except HT-29, K-562 and MOLT-4. This product does not show any reactivity to Mouse and Rat tissues. Whole Cell Extract-WCL (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), Hep G2 (Lane 3), HCT 116 (Lane 4), HT-29 (Lane 5), K-562 (Lane 6), MOLT-4 (Lane 7), Mouse Heart (Lane 8) and Rat Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A band around 25 kDa (*) corresponds to tissue IgG in mouse tissues.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of WWTR1 in PC-3 cells using WWTR1 Monoclonal Antibody (CL0370) (Product # MA5-24603), showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
