PA1-46190

antibody from Invitrogen Antibodies

Targeting: WWTR1 DKFZp586I1419, TAZ

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA1-46190

Provider

Invitrogen Antibodies

Product name

WWTR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Suggested positive control: 293 whole cell lysate, antigen standard for WWTR1 (transient overexpression lysate), HEK 293 lysate and kidney tissue lysate, human kidney protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of WWTR1 in HepG2 cells. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190). Nuclei (Blue) are counterstained with Hoecsht 33258.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of WWTR1 in HepG2 cells. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190). Nuclei (Blue) are counterstained with Hoecsht 33258.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of WWTR1 in formalin fixed paraffin-embedded (FFPE) human kidney. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.