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LearnPA1-46190
antibody from Invitrogen Antibodies
Targeting: WWTR1
DKFZp586I1419, TAZ
Western blot Immunocytochemistry
Immunoprecipitation
Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA1-46190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WWTR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: 293 whole cell lysate, antigen standard for WWTR1 (transient overexpression lysate), HEK 293 lysate and kidney tissue lysate, human kidney protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of PA1-46190 protein on HEK 293 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in HEK 293 cell lysate. Sample was incubated in WWTR1 polyclonal antibody (Product # PA1-46190).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in 0.5 mg/mL HeLa lysate. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HL-60 (Lane 1), K-562 (Lane 2), MOLT-4 (Lane 3), U2 OS (Lane 4), HeLa (Lane 5), Panc1 (Lane 6) and A431 (Lane 7). The blot was probed with Anti-WWTR1 Polyclonal Antibody (Product # PA1-46190, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 55kDa band corresponding to WWTR1 was seen in all cell lines tested except HL-60, K-562 and MOLT-4.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of WWTR1 in HepG2 cells. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190). Nuclei (Blue) are counterstained with Hoecsht 33258.
