PA5-72221

antibody from Invitrogen Antibodies

Targeting: AVPR2 DIR, DIR3, V2R

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72221

Provider

Invitrogen Antibodies

Product name

AVPR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AVPR2 in various lysates. Samples were incubated with AVPR2 polyclonal antibody (Product # PA5-72221) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Hela whole cell lysates; Lane 2: Jurkat whole cell lysates. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of AVPR2 in Jurkat cells (green). The cells were fixed with 4% paraformaldhyde (10 min) and permeabilized with 90% methanol (10 min). The sample was then incubated with 2% BSA followed by a AVPR2 polyclonal antibody (Product # PA5-72221) using a dilution of 1:25 for 60 min at 37°C. The secondary antibody used was with a dilution of 1:400 for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10^6 cells) used under the same conditions with an acquisition of >10, 000 events.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of (overlay histogram) of AVPR2 in Jurkat cells (green line). Samples were incubated with AVPR2 polyclonal antibody (Product # PA5-72221) using a dilution of 1:25 dilution for 60 min at 37°C followed by Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1:400 dilution for 40 min at 37°C. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.