LS-B10409

antibody from LSBio

Targeting: RHO CSNBAD1, OPN2, RP4

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10409

Provider

LSBio

Product name

IHC-plus™ Rhodopsin / RHO Antibody (clone A531) LS-B10409

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

A531

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Blot of bovine retinal extracts probed with Rhodopsin / RHO antibody. The antibody stains a band corresponding to retinal rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the rhodopsin protein.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Pig retinal section stained with Rhodopsin / RHO antibody (green) and counterstained with rabbit polyclonal antibody to neurofilament RPCA-NF-M (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).