MA1-722

antibody from Invitrogen Antibodies

Targeting: RHO CSNBAD1, OPN2, RP4

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA1-722 - Provider product page
Provider: Invitrogen Antibodies
Product name: Rhodopsin Monoclonal Antibody (1D4)
Antibody type: Monoclonal
Antigen: Other
Description: MA1-722 detects rhodopsin from human, mouse and bovine retinal samples. Clone 1D4 has also been used successfully in Zebrafish. It does not recognize rhodopsin in zebrafish rods, but instead labels long double cone outer segments that express red opsin (Yin, J. et al, 2012). MA1-722 has been successfully used in Western blot, IHC (P), immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~40 kDa protein representing rhodopsin from Sf9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments. The MA1-722 immunogen is bovine rhodopsin. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
Reactivity: Human, Mouse, Rat, Bovine
Host: Mouse
Isotype: IgG
Antibody clone number: 1D4
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

RHO protein structure - MA1-722 shown in red.

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 4-PBA suppressed light-induced photoreceptor histological changes and degeneration. ( A ) Representative images of immunohistochemistry for rhodopsin (green) counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue) 4 days after light exposure with or without 4-PBA treatment. Reduction in ONL thickness ( B ) and OS length ( C ) were suppressed by 4-PBA. LE, light exposure; Veh, vehicle; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segment. Data are presented as mean +- standard deviation. n = 8 in each group. Scale bar, 25 mum. * p < 0.05, ** p < 0.01, *** p < 0.001. ANOVA with Tukey post-hoc tests.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Protective effect of PBA in the photoreceptors of P23H RP models detected before photoreceptor loss ( A , A' ) Immunohistochemistry for Tom20 (green) and rhodopsin (pink) in the retina of P23H RP models at the age of 4 weeks with or without PBA treatment. Nuclei were counterstained with DAPI (blue). ( A ') Magnified images of IS and OS. IS area ( B ), and mRNA levels of rhodopsin ( C ) and crx ( D ) in the retina were already different with or without PBA treatment. Data are shown as mean +- standard deviation. Respective n for P23H RP models treated with vehicle and PBA, ( B ) 4 and 5; ( C ) 5 and 4; ( D ) 10 and 10. ONL, outer nuclear layer; IS, inner segments; OS, outer segments. * p < 0.05. Scale bar, ( A ) 20 mum; ( B ) 10 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Detection of rhodopsin levels in the retinas of all three mouse groups. (A) Western blot analysis was performed at P15 and the retinal protein extracts were run in SDS polyacrylamide gel and transferred to membrane. Images of membrane probed with anti-rhodopsin (upper) and anti-actin (lower) antibodies are shown. (B) The level of rhodopsin was calculated in all three groups. (C) Rhodopsin staining of retinal sections from the three groups at P15. Red, rhodopsin; blue, DAPI. Error bars: SEM. Statistical significance denoted by *P < 0.05, **P < 0.01, ****P < 0.001.