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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NBP1-47602 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47602, RRID:AB_10010560
- Product name
- Mouse Monoclonal Rhodopsin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
- Reactivity
- Human, Mouse, Bovine
- Host
- Mouse
- Antigen sequence
binds to the C- terminal epitope-T-
E-T-S-Q-V-A-P-A- (COOH) of rhodopsi
n- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Lack of Overt Retinal Degeneration in a K42E Dhdds Knock-In Mouse Model of RP59.
Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina.
Ramachandra Rao S, Fliesler SJ, Kotla P, Nguyen MN, Pittler SJ
Cells 2020 Apr 7;9(4)
Cells 2020 Apr 7;9(4)
Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina.
Cubizolle A, Guillou L, Mollereau B, Hamel CP, Brabet P
PloS one 2017;12(7):e0180148
PloS one 2017;12(7):e0180148
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
