Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NB600-469 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-469, RRID:AB_10001216
- Product name
- Rabbit Polyclonal URM1 Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography. Urm 1
- Reactivity
- Yeast
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.5 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: URM1 Antibody [NB600-469] - Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by western blot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Urm1 using a 1:1,000 dilution of IgG fraction of Rabbit-anti- Urm1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C.