Explore
Validate
LearnPA5-109422
antibody from Invitrogen Antibodies
Targeting: ASZ1
ALP1, ANKL1, C7orf7, CT1.19, GASZ, Orf3
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-109422 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASZ1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAASALRGLP VAGGGESSES EDDGWEIGYL DRTSQKLKRL LPIEEKKEKF KKAMTIGDVS LVQELLDSGI SVDSNFQYGW TPLMYAASVA NAELVRVLLD RGANASFEKD KQSILITACS AHGSEEQILK CVELLLSRNA DPNVACRRLM TPIMYAARDG HTQVVALLVA HGAEVNTQDE NGYTALTWAA RQGHKNIVLK LLELGANKML QTKDGKMPSE IAKRNKHHEI FNLLSFTLNP LEGKLQQLTK EDTICKILTT DSDREKDHIF SSYTAFGDLE VFLHGLGLEH MTDLLKERDI TLRHLLTMRE DEFTKNGITS KDQQKILAAL KELQVEEIQF GELSEETKLE
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.01 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASZ1 in extracts of various cell lines. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ALP1 in mouse testis. Samples were incubated in polyclonal ALP1 antibody (Product # PA5-109422) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ALP1 in rat testis. Samples were incubated in polyclonal ALP1 antibody (Product # PA5-109422) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ASZ1 in rat testis cells. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ASZ1 in mouse testis cells. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASZ1 in paraffin-embedded mouse testis. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASZ1 in paraffin-embedded rat testis. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASZ1 in paraffin-embedded rat testis. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASZ1 in paraffin-embedded mouse testis. Samples were incubated with ASZ1 Polyclonal antibody (Product # PA5-109422) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
