Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4246 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CEACAM-8/CD66b Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human CEACAM-8/CD66b in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P31997
- Isotype
- IgG
- Antibody clone number
- 913542
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance.
Zhang D, Zhou J, Tang D, Zhou L, Chou L, Chou KY, Tao L, Lu LM
Clinical immunology (Orlando, Fla.) 2017 Feb;175:34-40
Clinical immunology (Orlando, Fla.) 2017 Feb;175:34-40
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CEACAM-8/CD66b by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) and human peripheral blood granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CEACAM-8/CD66b in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).