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- Flow cytometry [2]
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- Product number
- DM1229 - Provider product page
- Provider
- OriGene
- Product name
- CD66b (CEACAM8) mouse monoclonal antibody, clone GM2H6, Purified
- Antibody type
- Monoclonal
- Description
- CD66b (CEACAM8) mouse monoclonal antibody, clone GM2H6, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM8
- Isotype
- IgG
- Antibody clone number
- GM2H6
- Vial size
- 100 µg
- Concentration
- 2.0 mg/ml
Submitted references CD66b regulates adhesion and activation of human eosinophils.
Yoon J, Terada A, Kita H
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8454-62
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8454-62
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.1 : FACS analysis of BOSC23 cells using GM2H6 antibody . BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (Red curve) or an irrelevant protein (control transfectant). Binding of GM-0512 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructswas tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8:Tet2; PSG: Brified GM-2H6 was tested on all CEACAM transfectants. A positive signal wasobtained only with CEACAM8 transfected cells (Red curve).
- Validation comment
- FC
