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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
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- Product number
- NBP2-54627 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM8/CD66b Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Clone 6/40c does not cross-react with human CEACAM1/3/4/5/6/7, rat CECAM1, or mouse CEACAM1/2.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEACAM8/CD66b Antibody (6/40c) [NBP2-54627] - Western blot analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM8 [6/40c] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CEACAM-8/CD66b Antibody (6/40c) [NBP2-54627] - Solid phase was coated with 3 ug/ml Anti-CEA binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM8 [6/40c] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CEACAM8/CD66b Antibody (6/40c) [NBP2-54627] - CEACAM-8/CD66b Antibody (6/40c) [NBP2-54627] - Analysis in human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM8 [6/40c]. Detection of the sample using 5 ug/ml pAb anti CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CEACAM-8/CD66b Antibody (6/40c) [NBP2-54627] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM8 [6/40c] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
