Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-0666-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD66b Monoclonal Antibody (G10F5), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This G10F5 monoclonal antibody reacts with human CD66b, one of four members of the CD66 family of granulocyte-specific activation antigens. Expressed on neutrophils and eosinophils, CD66b is upregulated following stimulation. This glycosyl-phosphatidylinositol-linked cell surface receptor plays a role in regulating neutrophil activation and adhesion to fibronectin, galectin-3, and E-selectin. Applications Reported: This G10F5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This G10F5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgM
- Antibody clone number
- G10F5
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Neuropeptides SP and CGRP Diminish the Moraxella catarrhalis Outer Membrane Vesicle- (OMV-) Triggered Inflammatory Response of Human A549 Epithelial Cells and Neutrophils.
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.
CD66b overexpression and homotypic aggregation of human peripheral blood neutrophils after activation by a gram-positive stimulus.
Intratumoral neutrophils and plasmacytoid dendritic cells indicate poor prognosis and are associated with pSTAT3 expression in AJCC stage I/II melanoma.
Impact of human granulocyte and monocyte isolation procedures on functional studies.
CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils.
Augustyniak D, Roszkowiak J, Wiśniewska I, Skała J, Gorczyca D, Drulis-Kawa Z
Mediators of inflammation 2018;2018:4847205
Mediators of inflammation 2018;2018:4847205
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.
Prodjinotho UF, von Horn C, Debrah AY, Batsa Debrah L, Albers A, Layland LE, Hoerauf A, Adjobimey T
PLoS neglected tropical diseases 2017 Jul;11(7):e0005777
PLoS neglected tropical diseases 2017 Jul;11(7):e0005777
CD66b overexpression and homotypic aggregation of human peripheral blood neutrophils after activation by a gram-positive stimulus.
Schmidt T, Zündorf J, Grüger T, Brandenburg K, Reiners AL, Zinserling J, Schnitzler N
Journal of leukocyte biology 2012 May;91(5):791-802
Journal of leukocyte biology 2012 May;91(5):791-802
Intratumoral neutrophils and plasmacytoid dendritic cells indicate poor prognosis and are associated with pSTAT3 expression in AJCC stage I/II melanoma.
Jensen TO, Schmidt H, Møller HJ, Donskov F, Høyer M, Sjoegren P, Christensen IJ, Steiniche T
Cancer 2012 May 1;118(9):2476-85
Cancer 2012 May 1;118(9):2476-85
Impact of human granulocyte and monocyte isolation procedures on functional studies.
Zhou L, Somasundaram R, Nederhof RF, Dijkstra G, Faber KN, Peppelenbosch MP, Fuhler GM
Clinical and vaccine immunology : CVI 2012 Jul;19(7):1065-74
Clinical and vaccine immunology : CVI 2012 Jul;19(7):1065-74
CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils.
Skubitz KM, Campbell KD, Skubitz AP
Journal of leukocyte biology 1996 Jul;60(1):106-17
Journal of leukocyte biology 1996 Jul;60(1):106-17
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgM Isotype Control PE (Product # 11-4754) (blue histogram) or Anti-Human CD66b PE (purple histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.
- Conjugate
- Green dye