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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 11-0666-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD66b Monoclonal Antibody (G10F5), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This G10F5 monoclonal antibody reacts with human CD66b, one of four members of the CD66 family of granulocyte-specific activation antigens. Expressed on neutrophils and eosinophils, CD66b is upregulated following stimulation. This glycosyl-phosphatidylinositol-linked cell surface receptor plays a role in regulating neutrophil activation and adhesion to fibronectin, galectin-3, and E-selectin. Applications Reported: This G10F5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This G10F5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgM
- Antibody clone number
- G10F5
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Platelets Fuel the Inflammasome Activation of Innate Immune Cells.
Loss of testosterone impairs anti-tumor neutrophil function.
A FACS-based approach to obtain viable eosinophils from human adipose tissue.
Immune Profile of the Nasal Mucosa in Patients with Cutaneous Leishmaniasis.
The Possible Role of Neutrophils in the Induction of Osteoclastogenesis.
Label-free optical hemogram of granulocytes enhanced by artificial neural networks.
The role of platelets in mediating a response to human influenza infection.
Tumor cell-released autophagosomes (TRAP) enhance apoptosis and immunosuppressive functions of neutrophils.
Neuropeptides SP and CGRP Diminish the Moraxella catarrhalis Outer Membrane Vesicle- (OMV-) Triggered Inflammatory Response of Human A549 Epithelial Cells and Neutrophils.
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.
CD66b overexpression and homotypic aggregation of human peripheral blood neutrophils after activation by a gram-positive stimulus.
Intratumoral neutrophils and plasmacytoid dendritic cells indicate poor prognosis and are associated with pSTAT3 expression in AJCC stage I/II melanoma.
Impact of human granulocyte and monocyte isolation procedures on functional studies.
CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils.
Rolfes V, Ribeiro LS, Hawwari I, Böttcher L, Rosero N, Maasewerd S, Santos MLS, Próchnicki T, Silva CMS, Wanderley CWS, Rothe M, Schmidt SV, Stunden HJ, Bertheloot D, Rivas MN, Fontes CJ, Carvalho LH, Cunha FQ, Latz E, Arditi M, Franklin BS
Cell reports 2020 May 12;31(6):107615
Cell reports 2020 May 12;31(6):107615
Loss of testosterone impairs anti-tumor neutrophil function.
Markman JL, Porritt RA, Wakita D, Lane ME, Martinon D, Noval Rivas M, Luu M, Posadas EM, Crother TR, Arditi M
Nature communications 2020 Mar 31;11(1):1613
Nature communications 2020 Mar 31;11(1):1613
A FACS-based approach to obtain viable eosinophils from human adipose tissue.
Hernandez JD, Tew BY, Li T, Gooden GC, Ghannam H, Masuda M, Madura J 2nd, Salhia B, Jacobsen EA, De Filippis E
Scientific reports 2020 Aug 6;10(1):13210
Scientific reports 2020 Aug 6;10(1):13210
Immune Profile of the Nasal Mucosa in Patients with Cutaneous Leishmaniasis.
Gómez-Zafra MJ, Navas A, Jojoa J, Murillo J, González C, Gómez MA
Infection and immunity 2020 Apr 20;88(5)
Infection and immunity 2020 Apr 20;88(5)
The Possible Role of Neutrophils in the Induction of Osteoclastogenesis.
Moonen CGJ, de Vries TJ, Rijkschroeff P, Poubelle PE, Nicu EA, Loos BG
Journal of immunology research 2019;2019:8672604
Journal of immunology research 2019;2019:8672604
Label-free optical hemogram of granulocytes enhanced by artificial neural networks.
Gupta RK, Chen M, Malcolm GPA, Hempler N, Dholakia K, Powis SJ
Optics express 2019 May 13;27(10):13706-13720
Optics express 2019 May 13;27(10):13706-13720
The role of platelets in mediating a response to human influenza infection.
Koupenova M, Corkrey HA, Vitseva O, Manni G, Pang CJ, Clancy L, Yao C, Rade J, Levy D, Wang JP, Finberg RW, Kurt-Jones EA, Freedman JE
Nature communications 2019 Apr 16;10(1):1780
Nature communications 2019 Apr 16;10(1):1780
Tumor cell-released autophagosomes (TRAP) enhance apoptosis and immunosuppressive functions of neutrophils.
Gao R, Ma J, Wen Z, Yang P, Zhao J, Xue M, Chen Y, Aldarouish M, Hu HM, Zhu XJ, Pan N, Wang LX
Oncoimmunology 2018;7(6):e1438108
Oncoimmunology 2018;7(6):e1438108
Neuropeptides SP and CGRP Diminish the Moraxella catarrhalis Outer Membrane Vesicle- (OMV-) Triggered Inflammatory Response of Human A549 Epithelial Cells and Neutrophils.
Augustyniak D, Roszkowiak J, Wiśniewska I, Skała J, Gorczyca D, Drulis-Kawa Z
Mediators of inflammation 2018;2018:4847205
Mediators of inflammation 2018;2018:4847205
Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes.
Prodjinotho UF, von Horn C, Debrah AY, Batsa Debrah L, Albers A, Layland LE, Hoerauf A, Adjobimey T
PLoS neglected tropical diseases 2017 Jul;11(7):e0005777
PLoS neglected tropical diseases 2017 Jul;11(7):e0005777
CD66b overexpression and homotypic aggregation of human peripheral blood neutrophils after activation by a gram-positive stimulus.
Schmidt T, Zündorf J, Grüger T, Brandenburg K, Reiners AL, Zinserling J, Schnitzler N
Journal of leukocyte biology 2012 May;91(5):791-802
Journal of leukocyte biology 2012 May;91(5):791-802
Intratumoral neutrophils and plasmacytoid dendritic cells indicate poor prognosis and are associated with pSTAT3 expression in AJCC stage I/II melanoma.
Jensen TO, Schmidt H, Møller HJ, Donskov F, Høyer M, Sjoegren P, Christensen IJ, Steiniche T
Cancer 2012 May 1;118(9):2476-85
Cancer 2012 May 1;118(9):2476-85
Impact of human granulocyte and monocyte isolation procedures on functional studies.
Zhou L, Somasundaram R, Nederhof RF, Dijkstra G, Faber KN, Peppelenbosch MP, Fuhler GM
Clinical and vaccine immunology : CVI 2012 Jul;19(7):1065-74
Clinical and vaccine immunology : CVI 2012 Jul;19(7):1065-74
CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils.
Skubitz KM, Campbell KD, Skubitz AP
Journal of leukocyte biology 1996 Jul;60(1):106-17
Journal of leukocyte biology 1996 Jul;60(1):106-17
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgM Isotype Control PE (Product # 11-4754) (blue histogram) or Anti-Human CD66b PE (purple histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Characterization of human blood from influenza-infected patients. a - d Blood from influenza-infected patients was fixed after intravenous collection and stained as described in Methods. In all cases DNA was assessed by DAPI. a Many platelets appeared similar to those observed in control blood from healthy donors with a size of 2-5 um. b Some platelets from influenza-infected patients had undergone spreading with a distinctive distribution of CD41. c Platelets and platelet microparticles associated with DNA (arrow). d Spread platelets were found surrounding released DNA with distinct DNA content in their center. Of note, blood from influenza-infected patients and controls in ( a - d ) was not permeabilized. Since formaldehyde can cause certain levels of permeabilization as a function of cross-linking positive staining in control samples for H4 and MPO do not necessarily indicate activation. e - g Levels of proteins related to DNA release in the plasma of influenza-infected patients assessed by ELISA. Source data are provided as a Source Data file. e neutrophil elastase, f myeloperoxidase (MPO), and g histone nucleosome core. The graphs represent the average +- SD of healthy donors ( n = 15) and influenza-infected patients ( n = 18); significance for ( e - g ) was assessed by Mann-Whitney U test, star symbol (*) indicates p < 0.0001. h , i To synchronize time of influenza presence as a function of infection and quantify the released DNA, we treated blood from human dono
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Analysis of neutrophils from control or androgen deprivation therapy (ADT) treated patients. a Percent of circulating neutrophils from control or ADT treated prostate cancer patients that have a mature, segmented appearance morphologically. b Percent Cd11b + CD66b + CD16 lo neutrophils. c Percent myeloid derived suppressor cell (MDSC)-like neutrophils defined as HLA-DR - CD14 - CD33 + CD15 + . d Percent fold change in migration (percent crystal violet positive area of transwell stimulated with a chemoattractant divided by percent area without chemoattractant). e Percent of unstimulated neutrophils producing DHR123. f Percent of neutrophils producing DHR123 following PMA stimulation. g Percent myeloperoxidase + (MPO + ) neutrophils. h Percent of plated netosising neutrophils at 1 and 3 h following purification. Data in a - h are mean +- s.e.m., * p < 0.05, ** p < 0.01, *** p < 0.001 by multivariable linear regression using ADT as its main predictor and adjusting for presence of prostatectomy, age, race, and current status; control prostate cancer patients ( n = 8 in a , c , g and n = 7 in b , d , e , f , h ) and prostate cancer pati e nts receiving ADT ( n = 22 in a , c , g and n = 21 in b , d , e , f , h ).
- Conjugate
- Green dye
