ALX-805-088-C100

antibody from Enzo Life Sciences

Targeting: CEACAM8 CD66b, CGM6

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-805-088-C100

Provider

Enzo Life Sciences

Product name

CEACAM8 (human) monoclonal antibody (GM2H6)

Antibody type

Monoclonal

Antigen

Other

Description

Protein G-affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

GM2H6

Vial size

100 μg

Storage

-20°C

Handling

For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Specificity testing by flow cytometry. Method: FACS analysis of BOSC23 cells using CEACAM8 (human), mAb (GM2H6) (Prod. No. ALX- 805-088). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of CEACAM8 (human), mAb (GM2H6) was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Antibody cross-reactivity with members of the CEA family. Method: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (green curves). An irrelevant monoclonal antibody served asa negative control (black curves). For specificity testing, CEACAM8 (human), mAb (GM2H6) (Prod. No. ALX- 805-088) was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).