Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 612306 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#612306, RRID:AB_2563460
- Product name
- Alexa Fluor® 594 anti-Cytochrome c
- Antibody type
- Monoclonal
- Antigen
- Rat cyt c-OVA
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 6H2.B4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 buffer for 10 minutes at room temperature and blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). Then the cells were stained with 5 µg/mL anti-Cytochrome c (clone 6H2.B4) Alexa Fluor® 594 overnight and Phalloidin Alexa Fluor® 488 (green) for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). This image was acquired with a 60X objective.
- Conjugate
- Yellow dye