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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- PA5-89973 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: YSHRMWGRLV GLVYILPAAY FWRKGWLSRG MKGRVLALCG LVCFQGLLGW YMVKSGLEEK SDSHDIPRVS QYRLAAHLGS ALVLYCASLW TSLSLLLPPH K; Positive Samples: U-251MG, A-431, Jurkat, Mouse heart, Mouse kidney, Mouse lung, Rat testis; Cellular Location: Mitochondrion membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.851 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX15 in extracts of various cell lines using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX15 in extracts of various cell lines using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COX15 was performed in L929 cells using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded rat spleen using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded rat heart using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded human lung cancer using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded human thyroid cancer using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded human placenta using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded mouse spleen using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COX15 in paraffin-embedded mouse heart using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100.
