PA5-89973

antibody from Invitrogen Antibodies

Targeting: COX15 CEMCOX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-89973

Provider

Invitrogen Antibodies

Product name

COX15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: YSHRMWGRLV GLVYILPAAY FWRKGWLSRG MKGRVLALCG LVCFQGLLGW YMVKSGLEEK SDSHDIPRVS QYRLAAHLGS ALVLYCASLW TSLSLLLPPH K; Positive Samples: U-251MG, A-431, Jurkat, Mouse heart, Mouse kidney, Mouse lung, Rat testis; Cellular Location: Mitochondrion membrane, Multi-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.851 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of COX15 in extracts of various cell lines using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of COX15 in extracts of various cell lines using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COX15 was performed in L929 cells using COX15 Polyclonal Antibody (Product # PA5-89973) at a dilution of 1:100. Blue: DAPI for nuclear staining.