612301

antibody from BioLegend

Targeting: COX15 CEMCOX2

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

612301

Provider

BioLegend

Proper citation

BioLegend Cat#612301, RRID:AB_315774

Product name

Purified anti-Cytochrome c

Antibody type

Monoclonal

Antigen

Rat cyt c-OVA

Description

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

Upon receipt, store at 4° C.

References

Submitted references

Adenovirus type 5 rupture of lysosomes leads to cathepsin B-dependent mitochondrial stress and production of reactive oxygen species.

McGuire KA, Barlan AU, Griffin TM, Wiethoff CM
Journal of virology 2011 Oct;85(20):10806-13

---

Molecular characterization of skeletal muscle atrophy in the R6/2 mouse model of Huntington's disease.

She P, Zhang Z, Marchionini D, Diaz WC, Jetton TJ, Kimball SR, Vary TC, Lang CH, Lynch CJ
American journal of physiology. Endocrinology and metabolism 2011 Jul;301(1):E49-61