PA5-29619

antibody from Invitrogen Antibodies

Targeting: TLR3 CD283

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29619

Provider

Invitrogen Antibodies

Product name

TLR3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: K562, THP-1.

Concentration

1.4 mg/mL

References

Submitted references

Myeloid Differentiation Primary Response 88-Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation.

Singh A, Devkar R, Basu A
Frontiers in oncology 2020;10:1780

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TLR3 absence confers increased survival with improved macrophage activity against pneumonia.

Suresh MV, Dolgachev VA, Zhang B, Balijepalli S, Swamy S, Mooliyil J, Kralovich G, Thomas B, Machado-Aranda D, Karmakar M, Lalwani S, Subramanian A, Anantharam A, Moore BB, Raghavendran K
JCI insight 2019 Dec 5;4(23)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TLR3/CD283 using 30 µg of A) K562 and B) THP-1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a TLR3/CD283 polyclonal antibody (Product # PA5-29619) at a dilution of 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of TLR3/CD283 in methanol-fixed HeLa cells using a TLR3/CD283 polyclonal antibody (Product # PA5-29619) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using TLR3 (Product # PA5-29619) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TLR3 Polyclonal Antibody detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. TLR3 stained by TLR3 Polyclonal Antibody (Product # PA5-29619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TLR3 Polyclonal Antibody detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. TLR3 stained by TLR3 Polyclonal Antibody (Product # PA5-29619) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Effect of MyD88 inhibitor on surface localization of TLR3. Fluorescent microscopy image of cells, treated with TLR3 ligand (10 mug/ml) with or without MyD88 inhibitor (1 muM) following immunocytochemical staining with antibody against TLR3 and Alexa 594-tagged secondary antibody and counterstained with DAPI. (A) T47D cell and (B) MDA-MB-231 cells. (C,D) Bar graph showing the localization of TLR3 in cell surface after observation through a microscope and analyses through the ImageJ package for all the experiment groups. The results are presented as mean +- SD ( p < 0.05 is treated as significant).