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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AFR-022-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Frizzled-2 (FZD2) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat heart lysate: - 1. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (1:200). 2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody , preincubated with Frizzled-2/FZD2 (extracellular) Blocking Peptide (#BLP-FR022).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MDA-231 breast carcinoma cell line lysate (lanes 1 and 3) and human THP-1 acute monocytic leukemia cell line lysate (lanes 2 and 4): - 1,2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (1:200).3,4. Anti-Frizzled-2 (FZD2) (extracellular) Antibody , preincubated with Frizzled-2/FZD2 (extracellular) Blocking Peptide (#BLP-FR022).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of FZD2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (2.5μg) + goat- Anti-rabbit-FITC.
