RQ6586

antibody from NSJ Bioreagents

Targeting: LSM8 YJR022W

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6586

Provider

NSJ Bioreagents

Product name

LSM8 Antibody

Antibody type

Monoclonal

Description

This highly specific LSM8 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

6B11

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the LSM8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HL60, 2) human Jurkat, 3) human ThP-1, 4) human K562, 5) rat testis, 6) rat pancreas, 7) mouse testis and 8) mouse pancreas tissue lysate with LSM8 antibody. Predicted molecular weight ~11 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HeLa cells with LSM8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colonic adenocarcinoma tissue with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human renal clear cell carcinoma tissue with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human spleen tissue with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human JK-1 cells with LSM8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSM8 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse RAW264.7 cells with LSM8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSM8 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat RH35 cells with LSM8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSM8 antibody.