NB200-112B

antibody from Novus Biologicals

Targeting: TSG101 TSG10, VPS23

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-112B

Provider

Novus Biologicals

Product name

Mouse Monoclonal TSG101 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Rat, Hamster, Simian

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The signal was developed with Trident femto Western HRP Substrate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug JC whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 10 % SDS-PAGE TSG101 antibody [4A10] dilution: 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE TSG101 antibody [4A10] dilution: 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: C2C12 10% SDS PAGE diluted at 1:500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Analysis of paraffin-embedded Colon carcinoma, using TSG101antibody at 1:100 dilution.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Detection of TSG101 using for capture at a concentration of 5 ug/mL and for detection at a concentration of 1.5 ug/mL.