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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00007251-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007251-M01, RRID:AB_548884
- Product name
- Mouse Monoclonal TSG101 Antibody
- Antibody type
- Monoclonal
- Description
- TSG101
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens.
Characterization of human thymic exosomes.
Notch signaling from the endosome requires a conserved dileucine motif.
Skogberg G, Lundberg V, Berglund M, Gudmundsdottir J, Telemo E, Lindgren S, Ekwall O
Immunology and cell biology 2015 Sep;93(8):727-34
Immunology and cell biology 2015 Sep;93(8):727-34
Characterization of human thymic exosomes.
Skogberg G, Gudmundsdottir J, van der Post S, Sandström K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O
PloS one 2013;8(7):e67554
PloS one 2013;8(7):e67554
Notch signaling from the endosome requires a conserved dileucine motif.
Zheng L, Saunders CA, Sorensen EB, Waxmonsky NC, Conner SD
Molecular biology of the cell 2013 Feb;24(3):297-307
Molecular biology of the cell 2013 Feb;24(3):297-307
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TSG101 Antibody (5B7) [H00007251-M01] - TSG101 expression in transfected 293T cell line by TSG101 monoclonal antibody (M01), clone 5B7. Lane 1: TSG101 transfected lysate (43.9 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TSG101 Antibody (5B7) [H00007251-M01] - TSG101 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TSG101 Antibody (5B7) [H00007251-M01] - Analysis of TSG101 over-expressed 293 cell line, cotransfected with TSG101 Validated Chimera RNAi ( Cat # H00007251-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TSG101 monoclonal antibody (M01), clone 5B7 (Cat # H00007251-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TSG101 Antibody (5B7) [H00007251-M01] - Detection limit for recombinant GST tagged TSG101 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TSG101 Antibody (5B7) [H00007251-M01] - Analysis of monoclonal antibody to TSG101 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TSG101 Antibody (5B7) [H00007251-M01] - Analysis of monoclonal antibody to TSG101 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
