A01233-2

antibody from Boster Biological Technology

Targeting: TSG101 TSG10, VPS23

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01233-2

Provider

Boster Biological Technology

Product name

Anti-TSG101 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for TSG101 detection. Tested with WB, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Serum exosomes derived from spontaneously hypertensive rats induce cardiac hypertrophy in vitro and in vivo by increasing autocrine release of angiotensin II in cardiomyocytes.

Yu J, Tang Y, Wang Y, Zhou M, Li Y, Hong J, Li C, Xu B, Guo X, Mao J
Biochemical pharmacology 2023 Apr;210:115462

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Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring SNHG7 to promote osteogenesis via TAF15-RUNX2 pathway.

Huang MZ, Chen HY, Peng GX, Sun H, Peng HC, Li HY, Liu XH, Li Q
Regenerative medicine 2022 Nov;17(11):819-833

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Exosome-mediated siRNA delivery to suppress postoperative breast cancer metastasis.

Zhao L, Gu C, Gan Y, Shao L, Chen H, Zhu H
Journal of controlled release : official journal of the Controlled Release Society 2020 Feb;318:1-15

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of TSG101 using anti-TSG101 antibody (A01233-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Hek293 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (Catalog # A01233-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44KD. The expected band size for TSG101 is at 44KD.

Additional image