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- References [2]
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- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- NBP1-80659 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80659, RRID:AB_11038777
- Product name
- Rabbit Polyclonal TSG101 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human TSG101 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer.
Active Wnt proteins are secreted on exosomes.
Pospichalova V, Svoboda J, Dave Z, Kotrbova A, Kaiser K, Klemova D, Ilkovics L, Hampl A, Crha I, Jandakova E, Minar L, Weinberger V, Bryja V
Journal of extracellular vesicles 2015;4:25530
Journal of extracellular vesicles 2015;4:25530
Active Wnt proteins are secreted on exosomes.
Gross JC, Chaudhary V, Bartscherer K, Boutros M
Nature cell biology 2012 Oct;14(10):1036-45
Nature cell biology 2012 Oct;14(10):1036-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human prostate shows moderate cytoplasmic positivity in glandular and in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
