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antibody from Novus Biologicals
Targeting: TSG101
TSG10, VPS23
Western blot ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [80]
- Comments [0]
- Validations
- Western blot [12]
- ELISA [1]
- Immunohistochemistry [4]
- Flow cytometry [3]
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- Product number
- NB200-112 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-112, RRID:AB_2256458
- Product name
- Mouse Monoclonal TSG101 Antibody
- Antibody type
- Monoclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR.
Syncytin 1 dependent horizontal transfer of marker genes from retrovirally transduced cells.
Extracellular Vesicle-derived circular RNAs confers chemoresistance in Colorectal cancer.
Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation.
Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse.
Exosomal transfer of mitochondria from airway myeloid-derived regulatory cells to T cells.
Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.
Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles.
Cavin-2 regulates the activity and stability of endothelial nitric-oxide synthase (eNOS) in angiogenesis.
A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes.
Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro.
Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.
Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid β (Aβ) production in the trans-Golgi network.
Regulation of the MVB pathway by SCAMP3.
Endosomal accumulation of the activated epidermal growth factor receptor (EGFR) induces apoptosis.
Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival.
Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport.
MARCH ubiquitin ligases alter the itinerary of clathrin-independent cargo from recycling to degradation.
Impaired β-amyloid secretion in Alzheimer's disease pathogenesis.
Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC.
The PTAP sequence within the p6 domain of human immunodeficiency virus type 1 Gag regulates its ubiquitination and MHC class I antigen presentation.
ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes.
Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation.
Quality control for unfolded proteins at the plasma membrane.
A targeted spatial-temporal proteomics approach implicates multiple cellular trafficking pathways in human cytomegalovirus virion maturation.
c-IAP1 and UbcH5 promote K11-linked polyubiquitination of RIP1 in TNF signalling.
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane.
Transferrin-directed internalization and cycling of transferrin receptor 2.
Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.
SCAMP3 negatively regulates epidermal growth factor receptor degradation and promotes receptor recycling.
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
In vitro budding of intralumenal vesicles into late endosomes is regulated by Alix and Tsg101.
Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4).
Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag.
The ESCRT-I subunit TSG101 controls endosome-to-cytosol release of viral RNA.
Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease.
Internalized antibodies to the Abeta domain of APP reduce neuronal Abeta and protect against synaptic alterations.
The molecular mechanism of hepcidin-mediated ferroportin down-regulation.
Up-regulation of tumor susceptibility gene 101 conveys poor prognosis through suppression of p21 expression in ovarian cancer.
Up-regulation of tumor susceptibility gene 101 protein in ovarian carcinomas revealed by proteomics analyses.
The ESCRT machinery is not required for human cytomegalovirus envelopment.
Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages.
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42.
An essential role for SNX1 in lysosomal sorting of protease-activated receptor-1: evidence for retromer-, Hrs-, and Tsg101-independent functions of sorting nexins.
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains.
Distinct roles for Tsg101 and Hrs in multivesicular body formation and inward vesiculation.
Beta-amyloid accumulation impairs multivesicular body sorting by inhibiting the ubiquitin-proteasome system.
Lysine-63-linked ubiquitination is required for endolysosomal degradation of class I molecules.
L-domain flanking sequences are important for host interactions and efficient budding of vesicular stomatitis virus recombinants.
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
Functional characterization of Ebola virus L-domains using VSV recombinants.
Alix regulates cortical actin and the spatial distribution of endosomes.
Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1.
The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation.
Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.
Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.
Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins.
Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes.
The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
Reduction of TSG101 protein has a negative impact on tumor cell growth.
The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
The protein network of HIV budding.
The protein network of HIV budding.
AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein.
Combination of microdissection and microarray analysis to identify gene expression changes between differentially located tumour cells in breast cancer.
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes.
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.
Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag.
Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag.
Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Minaidou A, Nicolaou P, Christodoulou K
PloS one 2019;14(2):e0211814
PloS one 2019;14(2):e0211814
Syncytin 1 dependent horizontal transfer of marker genes from retrovirally transduced cells.
Uygur B, Melikov K, Arakelyan A, Margolis LB, Chernomordik LV
Scientific reports 2019 Nov 27;9(1):17637
Scientific reports 2019 Nov 27;9(1):17637
Extracellular Vesicle-derived circular RNAs confers chemoresistance in Colorectal cancer.
Hon KW, Ab-Mutalib NS, Abdullah NMA, Jamal R, Abu N
Scientific reports 2019 Nov 11;9(1):16497
Scientific reports 2019 Nov 11;9(1):16497
Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation.
Murdica V, Giacomini E, Alteri A, Bartolacci A, Cermisoni GC, Zarovni N, Papaleo E, Montorsi F, Salonia A, Viganò P, Vago R
Fertility and sterility 2019 May;111(5):897-908.e2
Fertility and sterility 2019 May;111(5):897-908.e2
Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse.
Kwon MH, Song KM, Limanjaya A, Choi MJ, Ghatak K, Nguyen NM, Ock J, Yin GN, Kang JH, Lee MR, Gho YS, Ryu JK, Suh JK
Scientific reports 2019 Dec 27;9(1):20072
Scientific reports 2019 Dec 27;9(1):20072
Exosomal transfer of mitochondria from airway myeloid-derived regulatory cells to T cells.
Hough KP, Trevor JL, Strenkowski JG, Wang Y, Chacko BK, Tousif S, Chanda D, Steele C, Antony VB, Dokland T, Ouyang X, Zhang J, Duncan SR, Thannickal VJ, Darley-Usmar VM, Deshane JS
Redox biology 2018 Sep;18:54-64
Redox biology 2018 Sep;18:54-64
Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.
Hough KP, Wilson LS, Trevor JL, Strenkowski JG, Maina N, Kim YI, Spell ML, Wang Y, Chanda D, Dager JR, Sharma NS, Curtiss M, Antony VB, Dransfield MT, Chaplin DD, Steele C, Barnes S, Duncan SR, Prasain JK, Thannickal VJ, Deshane JS
Scientific reports 2018 Jul 9;8(1):10340
Scientific reports 2018 Jul 9;8(1):10340
Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles.
Guix FX, Corbett GT, Cha DJ, Mustapic M, Liu W, Mengel D, Chen Z, Aikawa E, Young-Pearse T, Kapogiannis D, Selkoe DJ, Walsh DM
International journal of molecular sciences 2018 Feb 27;19(3)
International journal of molecular sciences 2018 Feb 27;19(3)
Cavin-2 regulates the activity and stability of endothelial nitric-oxide synthase (eNOS) in angiogenesis.
Boopathy GTK, Kulkarni M, Ho SY, Boey A, Chua EWM, Barathi VA, Carney TJ, Wang X, Hong W
The Journal of biological chemistry 2017 Oct 27;292(43):17760-17776
The Journal of biological chemistry 2017 Oct 27;292(43):17760-17776
A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes.
Gschweitl M, Ulbricht A, Barnes CA, Enchev RI, Stoffel-Studer I, Meyer-Schaller N, Huotari J, Yamauchi Y, Greber UF, Helenius A, Peter M
eLife 2016 Mar 23;5:e13841
eLife 2016 Mar 23;5:e13841
Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro.
Shabbir A, Cox A, Rodriguez-Menocal L, Salgado M, Van Badiavas E
Stem cells and development 2015 Jul 15;24(14):1635-47
Stem cells and development 2015 Jul 15;24(14):1635-47
Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.
Nabhan JF, Hu R, Oh RS, Cohen SN, Lu Q
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 13;109(11):4146-51
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 13;109(11):4146-51
Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid β (Aβ) production in the trans-Golgi network.
Choy RW, Cheng Z, Schekman R
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):E2077-82
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):E2077-82
Regulation of the MVB pathway by SCAMP3.
Falguières T, Castle D, Gruenberg J
Traffic (Copenhagen, Denmark) 2012 Jan;13(1):131-42
Traffic (Copenhagen, Denmark) 2012 Jan;13(1):131-42
Endosomal accumulation of the activated epidermal growth factor receptor (EGFR) induces apoptosis.
Rush JS, Quinalty LM, Engelman L, Sherry DM, Ceresa BP
The Journal of biological chemistry 2012 Jan 2;287(1):712-22
The Journal of biological chemistry 2012 Jan 2;287(1):712-22
Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival.
Toyofuku T, Nojima S, Ishikawa T, Takamatsu H, Tsujimura T, Uemura A, Matsuda J, Seki T, Kumanogoh A
Genes & development 2012 Apr 15;26(8):816-29
Genes & development 2012 Apr 15;26(8):816-29
Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport.
Pasqual G, Rojek JM, Masin M, Chatton JY, Kunz S
PLoS pathogens 2011 Sep;7(9):e1002232
PLoS pathogens 2011 Sep;7(9):e1002232
MARCH ubiquitin ligases alter the itinerary of clathrin-independent cargo from recycling to degradation.
Eyster CA, Cole NB, Petersen S, Viswanathan K, Früh K, Donaldson JG
Molecular biology of the cell 2011 Sep;22(17):3218-30
Molecular biology of the cell 2011 Sep;22(17):3218-30
Impaired β-amyloid secretion in Alzheimer's disease pathogenesis.
Tampellini D, Rahman N, Lin MT, Capetillo-Zarate E, Gouras GK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Oct 26;31(43):15384-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Oct 26;31(43):15384-90
Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC.
Battke C, Ruiss R, Welsch U, Wimberger P, Lang S, Jochum S, Zeidler R
Cancer immunology, immunotherapy : CII 2011 May;60(5):639-48
Cancer immunology, immunotherapy : CII 2011 May;60(5):639-48
The PTAP sequence within the p6 domain of human immunodeficiency virus type 1 Gag regulates its ubiquitination and MHC class I antigen presentation.
Hahn S, Setz C, Wild J, Schubert U
Journal of immunology (Baltimore, Md. : 1950) 2011 May 15;186(10):5706-18
Journal of immunology (Baltimore, Md. : 1950) 2011 May 15;186(10):5706-18
ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes.
Balut CM, Gao Y, Murray SA, Thibodeau PH, Devor DC
American journal of physiology. Cell physiology 2010 Nov;299(5):C1015-27
American journal of physiology. Cell physiology 2010 Nov;299(5):C1015-27
Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation.
Hayer A, Stoeber M, Ritz D, Engel S, Meyer HH, Helenius A
The Journal of cell biology 2010 Nov 1;191(3):615-29
The Journal of cell biology 2010 Nov 1;191(3):615-29
Quality control for unfolded proteins at the plasma membrane.
Apaja PM, Xu H, Lukacs GL
The Journal of cell biology 2010 Nov 1;191(3):553-70
The Journal of cell biology 2010 Nov 1;191(3):553-70
A targeted spatial-temporal proteomics approach implicates multiple cellular trafficking pathways in human cytomegalovirus virion maturation.
Moorman NJ, Sharon-Friling R, Shenk T, Cristea IM
Molecular & cellular proteomics : MCP 2010 May;9(5):851-60
Molecular & cellular proteomics : MCP 2010 May;9(5):851-60
c-IAP1 and UbcH5 promote K11-linked polyubiquitination of RIP1 in TNF signalling.
Dynek JN, Goncharov T, Dueber EC, Fedorova AV, Izrael-Tomasevic A, Phu L, Helgason E, Fairbrother WJ, Deshayes K, Kirkpatrick DS, Vucic D
The EMBO journal 2010 Dec 15;29(24):4198-209
The EMBO journal 2010 Dec 15;29(24):4198-209
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
Inuzuka T, Suzuki H, Kawasaki M, Shibata H, Wakatsuki S, Maki M
BMC structural biology 2010 Aug 6;10:25
BMC structural biology 2010 Aug 6;10:25
ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane.
Truschel ST, Simoes S, Setty SR, Harper DC, Tenza D, Thomas PC, Herman KE, Sackett SD, Cowan DC, Theos AC, Raposo G, Marks MS
Traffic (Copenhagen, Denmark) 2009 Sep;10(9):1318-36
Traffic (Copenhagen, Denmark) 2009 Sep;10(9):1318-36
Transferrin-directed internalization and cycling of transferrin receptor 2.
Chen J, Wang J, Meyers KR, Enns CA
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1488-501
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1488-501
Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.
Leithe E, Kjenseth A, Sirnes S, Stenmark H, Brech A, Rivedal E
Journal of cell science 2009 Nov 1;122(Pt 21):3883-93
Journal of cell science 2009 Nov 1;122(Pt 21):3883-93
SCAMP3 negatively regulates epidermal growth factor receptor degradation and promotes receptor recycling.
Aoh QL, Castle AM, Hubbard CH, Katsumata O, Castle JD
Molecular biology of the cell 2009 Mar;20(6):1816-32
Molecular biology of the cell 2009 Mar;20(6):1816-32
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
Glozman R, Okiyoneda T, Mulvihill CM, Rini JM, Barriere H, Lukacs GL
The Journal of cell biology 2009 Mar 23;184(6):847-62
The Journal of cell biology 2009 Mar 23;184(6):847-62
Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
Okumura M, Ichioka F, Kobayashi R, Suzuki H, Yoshida H, Shibata H, Maki M
Biochemical and biophysical research communications 2009 Aug 14;386(1):237-41
Biochemical and biophysical research communications 2009 Aug 14;386(1):237-41
In vitro budding of intralumenal vesicles into late endosomes is regulated by Alix and Tsg101.
Falguières T, Luyet PP, Bissig C, Scott CC, Velluz MC, Gruenberg J
Molecular biology of the cell 2008 Nov;19(11):4942-55
Molecular biology of the cell 2008 Nov;19(11):4942-55
Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4).
Ichioka F, Kobayashi R, Katoh K, Shibata H, Maki M
The FEBS journal 2008 Feb;275(4):682-92
The FEBS journal 2008 Feb;275(4):682-92
Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag.
Valiathan RR, Resh MD
Virology journal 2008 Feb 11;5:23
Virology journal 2008 Feb 11;5:23
The ESCRT-I subunit TSG101 controls endosome-to-cytosol release of viral RNA.
Luyet PP, Falguières T, Pons V, Pattnaik AK, Gruenberg J
Traffic (Copenhagen, Denmark) 2008 Dec;9(12):2279-90
Traffic (Copenhagen, Denmark) 2008 Dec;9(12):2279-90
Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.
Shibata H, Suzuki H, Kakiuchi T, Inuzuka T, Yoshida H, Mizuno T, Maki M
The Journal of biological chemistry 2008 Apr 11;283(15):9623-32
The Journal of biological chemistry 2008 Apr 11;283(15):9623-32
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease.
Filimonenko M, Stuffers S, Raiborg C, Yamamoto A, Malerød L, Fisher EM, Isaacs A, Brech A, Stenmark H, Simonsen A
The Journal of cell biology 2007 Nov 5;179(3):485-500
The Journal of cell biology 2007 Nov 5;179(3):485-500
Internalized antibodies to the Abeta domain of APP reduce neuronal Abeta and protect against synaptic alterations.
Tampellini D, Magrané J, Takahashi RH, Li F, Lin MT, Almeida CG, Gouras GK
The Journal of biological chemistry 2007 Jun 29;282(26):18895-906
The Journal of biological chemistry 2007 Jun 29;282(26):18895-906
The molecular mechanism of hepcidin-mediated ferroportin down-regulation.
De Domenico I, Ward DM, Langelier C, Vaughn MB, Nemeth E, Sundquist WI, Ganz T, Musci G, Kaplan J
Molecular biology of the cell 2007 Jul;18(7):2569-78
Molecular biology of the cell 2007 Jul;18(7):2569-78
Up-regulation of tumor susceptibility gene 101 conveys poor prognosis through suppression of p21 expression in ovarian cancer.
Young TW, Rosen DG, Mei FC, Li N, Liu J, Wang XF, Cheng X
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jul 1;13(13):3848-54
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jul 1;13(13):3848-54
Up-regulation of tumor susceptibility gene 101 protein in ovarian carcinomas revealed by proteomics analyses.
Young TW, Mei FC, Rosen DG, Yang G, Li N, Liu J, Cheng X
Molecular & cellular proteomics : MCP 2007 Feb;6(2):294-304
Molecular & cellular proteomics : MCP 2007 Feb;6(2):294-304
The ESCRT machinery is not required for human cytomegalovirus envelopment.
Fraile-Ramos A, Pelchen-Matthews A, Risco C, Rejas MT, Emery VC, Hassan-Walker AF, Esteban M, Marsh M
Cellular microbiology 2007 Dec;9(12):2955-67
Cellular microbiology 2007 Dec;9(12):2955-67
Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages.
Chertova E, Chertov O, Coren LV, Roser JD, Trubey CM, Bess JW Jr, Sowder RC 2nd, Barsov E, Hood BL, Fisher RJ, Nagashima K, Conrads TP, Veenstra TD, Lifson JD, Ott DE
Journal of virology 2006 Sep;80(18):9039-52
Journal of virology 2006 Sep;80(18):9039-52
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
Langelier C, von Schwedler UK, Fisher RD, De Domenico I, White PL, Hill CP, Kaplan J, Ward D, Sundquist WI
Journal of virology 2006 Oct;80(19):9465-80
Journal of virology 2006 Oct;80(19):9465-80
YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42.
Hui EK, Barman S, Tang DH, France B, Nayak DP
Journal of virology 2006 Mar;80(5):2291-308
Journal of virology 2006 Mar;80(5):2291-308
An essential role for SNX1 in lysosomal sorting of protease-activated receptor-1: evidence for retromer-, Hrs-, and Tsg101-independent functions of sorting nexins.
Gullapalli A, Wolfe BL, Griffin CT, Magnuson T, Trejo J
Molecular biology of the cell 2006 Mar;17(3):1228-38
Molecular biology of the cell 2006 Mar;17(3):1228-38
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
Bache KG, Stuffers S, Malerød L, Slagsvold T, Raiborg C, Lechardeur D, Wälchli S, Lukacs GL, Brech A, Stenmark H
Molecular biology of the cell 2006 Jun;17(6):2513-23
Molecular biology of the cell 2006 Jun;17(6):2513-23
Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains.
Raiborg C, Wesche J, Malerød L, Stenmark H
Journal of cell science 2006 Jun 15;119(Pt 12):2414-24
Journal of cell science 2006 Jun 15;119(Pt 12):2414-24
Distinct roles for Tsg101 and Hrs in multivesicular body formation and inward vesiculation.
Razi M, Futter CE
Molecular biology of the cell 2006 Aug;17(8):3469-83
Molecular biology of the cell 2006 Aug;17(8):3469-83
Beta-amyloid accumulation impairs multivesicular body sorting by inhibiting the ubiquitin-proteasome system.
Almeida CG, Takahashi RH, Gouras GK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 19;26(16):4277-88
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 19;26(16):4277-88
Lysine-63-linked ubiquitination is required for endolysosomal degradation of class I molecules.
Duncan LM, Piper S, Dodd RB, Saville MK, Sanderson CM, Luzio JP, Lehner PJ
The EMBO journal 2006 Apr 19;25(8):1635-45
The EMBO journal 2006 Apr 19;25(8):1635-45
L-domain flanking sequences are important for host interactions and efficient budding of vesicular stomatitis virus recombinants.
Irie T, Harty RN
Journal of virology 2005 Oct;79(20):12617-22
Journal of virology 2005 Oct;79(20):12617-22
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J
The Journal of biological chemistry 2005 Mar 18;280(11):10548-55
The Journal of biological chemistry 2005 Mar 18;280(11):10548-55
Functional characterization of Ebola virus L-domains using VSV recombinants.
Irie T, Licata JM, Harty RN
Virology 2005 Jun 5;336(2):291-8
Virology 2005 Jun 5;336(2):291-8
Alix regulates cortical actin and the spatial distribution of endosomes.
Cabezas A, Bache KG, Brech A, Stenmark H
Journal of cell science 2005 Jun 15;118(Pt 12):2625-35
Journal of cell science 2005 Jun 15;118(Pt 12):2625-35
Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1.
Lin Y, Kimpler LA, Naismith TV, Lauer JM, Hanson PI
The Journal of biological chemistry 2005 Apr 1;280(13):12799-809
The Journal of biological chemistry 2005 Apr 1;280(13):12799-809
The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation.
Bache KG, Slagsvold T, Cabezas A, Rosendal KR, Raiborg C, Stenmark H
Molecular biology of the cell 2004 Sep;15(9):4337-46
Molecular biology of the cell 2004 Sep;15(9):4337-46
Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.
Irie T, Licata JM, McGettigan JP, Schnell MJ, Harty RN
Journal of virology 2004 Mar;78(6):2657-65
Journal of virology 2004 Mar;78(6):2657-65
Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.
Irie T, Licata JM, McGettigan JP, Schnell MJ, Harty RN
Journal of virology 2004 Mar;78(6):2657-65
Journal of virology 2004 Mar;78(6):2657-65
Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins.
Heissmeyer V, Macián F, Im SH, Varma R, Feske S, Venuprasad K, Gu H, Liu YC, Dustin ML, Rao A
Nature immunology 2004 Mar;5(3):255-65
Nature immunology 2004 Mar;5(3):255-65
Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes.
Sharma M, Pampinella F, Nemes C, Benharouga M, So J, Du K, Bache KG, Papsin B, Zerangue N, Stenmark H, Lukacs GL
The Journal of cell biology 2004 Mar 15;164(6):923-33
The Journal of cell biology 2004 Mar 15;164(6):923-33
The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist WI
The Journal of biological chemistry 2004 Aug 20;279(34):36059-71
The Journal of biological chemistry 2004 Aug 20;279(34):36059-71
Reduction of TSG101 protein has a negative impact on tumor cell growth.
Zhu G, Gilchrist R, Borley N, Chng HW, Morgan M, Marshall JF, Camplejohn RS, Muir GH, Hart IR
International journal of cancer 2004 Apr 20;109(4):541-7
International journal of cancer 2004 Apr 20;109(4):541-7
The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
Gottwein E, Bodem J, Müller B, Schmechel A, Zentgraf H, Kräusslich HG
Journal of virology 2003 Sep;77(17):9474-85
Journal of virology 2003 Sep;77(17):9474-85
The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
Gottwein E, Bodem J, Müller B, Schmechel A, Zentgraf H, Kräusslich HG
Journal of virology 2003 Sep;77(17):9474-85
Journal of virology 2003 Sep;77(17):9474-85
The protein network of HIV budding.
von Schwedler UK, Stuchell M, Müller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, Scott A, Kräusslich HG, Kaplan J, Morham SG, Sundquist WI
Cell 2003 Sep 19;114(6):701-13
Cell 2003 Sep 19;114(6):701-13
The protein network of HIV budding.
von Schwedler UK, Stuchell M, Müller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, Scott A, Kräusslich HG, Kaplan J, Morham SG, Sundquist WI
Cell 2003 Sep 19;114(6):701-13
Cell 2003 Sep 19;114(6):701-13
AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
Strack B, Calistri A, Craig S, Popova E, Göttlinger HG
Cell 2003 Sep 19;114(6):689-99
Cell 2003 Sep 19;114(6):689-99
AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
Strack B, Calistri A, Craig S, Popova E, Göttlinger HG
Cell 2003 Sep 19;114(6):689-99
Cell 2003 Sep 19;114(6):689-99
Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein.
Chiou CT, Hu CA, Chen PH, Liao CL, Lin YL, Wang JJ
The Journal of general virology 2003 Oct;84(Pt 10):2795-2805
The Journal of general virology 2003 Oct;84(Pt 10):2795-2805
Combination of microdissection and microarray analysis to identify gene expression changes between differentially located tumour cells in breast cancer.
Zhu G, Reynolds L, Crnogorac-Jurcevic T, Gillett CE, Dublin EA, Marshall JF, Barnes D, D'Arrigo C, Van Trappen PO, Lemoine NR, Hart IR
Oncogene 2003 Jun 12;22(24):3742-8
Oncogene 2003 Jun 12;22(24):3742-8
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes.
Bache KG, Brech A, Mehlum A, Stenmark H
The Journal of cell biology 2003 Aug 4;162(3):435-42
The Journal of cell biology 2003 Aug 4;162(3):435-42
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.
Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist WI
The Journal of cell biology 2003 Aug 4;162(3):425-34
The Journal of cell biology 2003 Aug 4;162(3):425-34
Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag.
Myers EL, Allen JF
Journal of virology 2002 Nov;76(22):11226-35
Journal of virology 2002 Nov;76(22):11226-35
Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag.
Myers EL, Allen JF
Journal of virology 2002 Nov;76(22):11226-35
Journal of virology 2002 Nov;76(22):11226-35
Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
Bishop N, Horman A, Woodman P
The Journal of cell biology 2002 Apr 1;157(1):91-101
The Journal of cell biology 2002 Apr 1;157(1):91-101
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist WI
Cell 2001 Oct 5;107(1):55-65
Cell 2001 Oct 5;107(1):55-65
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist WI
Cell 2001 Oct 5;107(1):55-65
Cell 2001 Oct 5;107(1):55-65
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Supportive validation
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The signal was developed with Trident Sharp-ECL.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: C2C12 10% SDS PAGE diluted at 1:500 Image using the Biotin form of this antibody.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - K562 whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
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- Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. Western blot analysis of exosomes revealed differential expression of TSG101 between HCT116-R exosomes and HCT116-P exosomes. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-019-53063-y), licensed under a CC-BY licence.
Supportive validation
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- ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.
Supportive validation
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- Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Paraffin-embedded Colon carcinoma, using antibody at 1:100 dilution.
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- Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 antibody [4A10] (NB200-112). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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- Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (NB200-112). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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- Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (NB200-112). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Supportive validation
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- Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on HeLa cells with TSG101 Antibody (4A10) NB200-112F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
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- Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on Hek293 cells with TSG101 Antibody [4A10] NB200-112PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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- Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] (NB200-112) detects TSG101 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: TSG101 antibody [4A10] (NB200-112). Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
