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Targeting: TSG101
TSG10, VPS23
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- References [123]
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- Western blot [10]
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- GTX70255 - Provider product page
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- GeneTex
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- GeneTex Cat#GTX70255, RRID:AB_373239
- Product name
- TSG101 antibody [4A10]
- Antibody type
- Monoclonal
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- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
Submitted references Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation.
Stimulated release of intraluminal vesicles from Weibel-Palade bodies.
Prostate cancer cells and exosomes in acidic condition show increased carbonic anhydrase IX expression and activity.
Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials.
Detection of exosomal tyrosine receptor kinase B as a potential biomarker in ovarian cancer.
SIMPLE binds specifically to PI4P through SIMPLE-like domain and participates in protein trafficking in the trans-Golgi network and/or recycling endosomes.
Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins.
Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules.
Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.
Tumor-derived extracellular vesicles activate primary monocytes.
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles.
Distinct repertoires of microRNAs present in mouse astrocytes compared to astrocyte-secreted exosomes.
Extracellular vesicles mediate signaling between the aqueous humor producing and draining cells in the ocular system.
Radiation alters the cargo of exosomes released from squamous head and neck cancer cells to promote migration of recipient cells.
Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA.
Tsg101 chaperone function revealed by HIV-1 assembly inhibitors.
Exosome secretion promotes chemotaxis of cancer cells.
Reciprocal regulation of γ-globin expression by exo-miRNAs: Relevance to γ-globin silencing in β-thalassemia major.
Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation.
Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy.
Enhanced exosome secretion in Down syndrome brain - a protective mechanism to alleviate neuronal endosomal abnormalities.
Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A.
Tetherin is an exosomal tether.
Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS.
ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.
Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway.
Cortactin promotes exosome secretion by controlling branched actin dynamics.
The Endocytic Fate of the Transferrin Receptor Is Regulated by c-Abl Kinase.
Y-box protein 1 is required to sort microRNAs into exosomes in cells and in a cell-free reaction.
Physical exercise induces rapid release of small extracellular vesicles into the circulation.
RAL-1 controls multivesicular body biogenesis and exosome secretion.
Cell-to-cell propagation of the bacterial toxin CNF1 via extracellular vesicles: potential impact on the therapeutic use of the toxin.
Translocation of interleukin-1β into a vesicle intermediate in autophagy-mediated secretion.
Extracellular vesicle sorting of α-Synuclein is regulated by sumoylation.
Exosomes released by keratinocytes modulate melanocyte pigmentation.
WASH and Tsg101/ALIX-dependent diversion of stress-internalized EGFR from the canonical endocytic pathway.
ESCRTs regulate amyloid precursor protein sorting in multivesicular bodies and intracellular amyloid-β accumulation.
Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites.
Spatial control of Shoc2-scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5.
Functional Interaction Between the ESCRT-I Component TSG101 and the HSV-1 Tegument Ubiquitin Specific Protease.
Vascular smooth muscle cell calcification is mediated by regulated exosome secretion.
The multivesicular body is the major internal site of prion conversion.
Septin6 and Septin7 GTP binding proteins regulate AP-3- and ESCRT-dependent multivesicular body biogenesis.
ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation.
ATP13A2/PARK9 regulates secretion of exosomes and α-synuclein.
Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer's disease.
ESCRT regulates surface expression of the Kir2.1 potassium channel.
Functional significance of macrophage-derived exosomes in inflammation and pain.
NDRG1 functions in LDL receptor trafficking by regulating endosomal recycling and degradation.
Identification of late assembly domains of the human endogenous retrovirus-K(HML-2).
Macrophage-derived matrix vesicles: an alternative novel mechanism for microcalcification in atherosclerotic plaques.
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.
Tsg101 interacts with herpes simplex virus 1 VP1/2 and is a substrate of VP1/2 ubiquitin-specific protease domain activity.
RAB7 and TSG101 are required for the constitutive recycling of unliganded EGFRs via distinct mechanisms.
Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles.
The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation.
Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation.
The ESCRT machinery is recruited by the viral BFRF1 protein to the nucleus-associated membrane for the maturation of Epstein-Barr Virus.
Regulation of the EGF transcriptional response by endocytic sorting.
Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.
Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid β (Aβ) production in the trans-Golgi network.
Regulation of the MVB pathway by SCAMP3.
Endosomal accumulation of the activated epidermal growth factor receptor (EGFR) induces apoptosis.
Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport.
MARCH ubiquitin ligases alter the itinerary of clathrin-independent cargo from recycling to degradation.
Impaired β-amyloid secretion in Alzheimer's disease pathogenesis.
The PTAP sequence within the p6 domain of human immunodeficiency virus type 1 Gag regulates its ubiquitination and MHC class I antigen presentation.
ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes.
Quality control for unfolded proteins at the plasma membrane.
A targeted spatial-temporal proteomics approach implicates multiple cellular trafficking pathways in human cytomegalovirus virion maturation.
c-IAP1 and UbcH5 promote K11-linked polyubiquitination of RIP1 in TNF signalling.
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane.
Transferrin-directed internalization and cycling of transferrin receptor 2.
Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.
SCAMP3 negatively regulates epidermal growth factor receptor degradation and promotes receptor recycling.
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
Hrs and SNX3 functions in sorting and membrane invagination within multivesicular bodies.
In vitro budding of intralumenal vesicles into late endosomes is regulated by Alix and Tsg101.
Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4).
Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag.
The ESCRT-I subunit TSG101 controls endosome-to-cytosol release of viral RNA.
Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.
MVB-12, a fourth subunit of metazoan ESCRT-I, functions in receptor downregulation.
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease.
Internalized antibodies to the Abeta domain of APP reduce neuronal Abeta and protect against synaptic alterations.
The molecular mechanism of hepcidin-mediated ferroportin down-regulation.
The ESCRT machinery is not required for human cytomegalovirus envelopment.
Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages.
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42.
An essential role for SNX1 in lysosomal sorting of protease-activated receptor-1: evidence for retromer-, Hrs-, and Tsg101-independent functions of sorting nexins.
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains.
Distinct roles for Tsg101 and Hrs in multivesicular body formation and inward vesiculation.
Beta-amyloid accumulation impairs multivesicular body sorting by inhibiting the ubiquitin-proteasome system.
Lysine-63-linked ubiquitination is required for endolysosomal degradation of class I molecules.
L-domain flanking sequences are important for host interactions and efficient budding of vesicular stomatitis virus recombinants.
The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B.
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
Functional characterization of Ebola virus L-domains using VSV recombinants.
Alix regulates cortical actin and the spatial distribution of endosomes.
Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1.
The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation.
Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.
Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins.
Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes.
The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
Reduction of TSG101 protein has a negative impact on tumor cell growth.
The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
The protein network of HIV budding.
AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein.
Combination of microdissection and microarray analysis to identify gene expression changes between differentially located tumour cells in breast cancer.
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes.
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.
Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag.
Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Wierz M, Pierson S, Gargiulo E, Guerin C, Moussay E, Paggetti J
Methods in molecular biology (Clifton, N.J.) 2019;1884:231-245
Methods in molecular biology (Clifton, N.J.) 2019;1884:231-245
Stimulated release of intraluminal vesicles from Weibel-Palade bodies.
Streetley J, Fonseca AV, Turner J, Kiskin NI, Knipe L, Rosenthal PB, Carter T
Blood 2019 Jun 20;133(25):2707-2717
Blood 2019 Jun 20;133(25):2707-2717
Prostate cancer cells and exosomes in acidic condition show increased carbonic anhydrase IX expression and activity.
Logozzi M, Capasso C, Di Raimo R, Del Prete S, Mizzoni D, Falchi M, Supuran CT, Fais S
Journal of enzyme inhibition and medicinal chemistry 2019 Dec;34(1):272-278
Journal of enzyme inhibition and medicinal chemistry 2019 Dec;34(1):272-278
Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials.
Logozzi M, Mizzoni D, Bocca B, Di Raimo R, Petrucci F, Caimi S, Alimonti A, Falchi M, Cappello F, Campanella C, Bavisotto CC, David S, Bucchieri F, Angelini DF, Battistini L, Fais S
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2019 Apr;137:23-36
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2019 Apr;137:23-36
Detection of exosomal tyrosine receptor kinase B as a potential biomarker in ovarian cancer.
Li W, Lu Y, Yu X, Yong M, Ma D, Gao Q
Journal of cellular biochemistry 2019 Apr;120(4):6361-6369
Journal of cellular biochemistry 2019 Apr;120(4):6361-6369
SIMPLE binds specifically to PI4P through SIMPLE-like domain and participates in protein trafficking in the trans-Golgi network and/or recycling endosomes.
Moriwaki Y, Ohno Y, Ishii T, Takamura Y, Kita Y, Watabe K, Sango K, Tsuji S, Misawa H
PloS one 2018;13(6):e0199829
PloS one 2018;13(6):e0199829
Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
Heisler FF, Pechmann Y, Wieser I, Altmeppen HC, Veenendaal L, Muhia M, Schweizer M, Glatzel M, Krasemann S, Kneussel M
Neuron 2018 Sep 19;99(6):1155-1169.e9
Neuron 2018 Sep 19;99(6):1155-1169.e9
Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins.
Winer H, Fraiberg M, Abada A, Dadosh T, Tamim-Yecheskel BC, Elazar Z
Nature communications 2018 Sep 14;9(1):3744
Nature communications 2018 Sep 14;9(1):3744
Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules.
Boussadia Z, Lamberti J, Mattei F, Pizzi E, Puglisi R, Zanetti C, Pasquini L, Fratini F, Fantozzi L, Felicetti F, Fecchi K, Raggi C, Sanchez M, D'Atri S, Carè A, Sargiacomo M, Parolini I
Journal of experimental & clinical cancer research : CR 2018 Oct 5;37(1):245
Journal of experimental & clinical cancer research : CR 2018 Oct 5;37(1):245
Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.
Novo D, Heath N, Mitchell L, Caligiuri G, MacFarlane A, Reijmer D, Charlton L, Knight J, Calka M, McGhee E, Dornier E, Sumpton D, Mason S, Echard A, Klinkert K, Secklehner J, Kruiswijk F, Vousden K, Macpherson IR, Blyth K, Bailey P, Yin H, Carlin LM, Morton J, Zanivan S, Norman JC
Nature communications 2018 Nov 29;9(1):5069
Nature communications 2018 Nov 29;9(1):5069
Tumor-derived extracellular vesicles activate primary monocytes.
Gärtner K, Battke C, Dünzkofer J, Hüls C, von Neubeck B, Kellner MK, Fiestas E, Fackler S, Lang S, Zeidler R
Cancer medicine 2018 May;7(5):2013-2020
Cancer medicine 2018 May;7(5):2013-2020
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
Gao K, Zhang Y, Shi Q, Zhang J, Zhang L, Sun H, Jiao D, Zhao X, Tao H, Wei Y, Wang Y, Saiyin H, Zhao SM, Li Y, Zhang P, Wang C
Cell death & disease 2018 May 1;9(5):528
Cell death & disease 2018 May 1;9(5):528
Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles.
Durcin M, Fleury A, Taillebois E, Hilairet G, Krupova Z, Henry C, Truchet S, Trötzmüller M, Köfeler H, Mabilleau G, Hue O, Andriantsitohaina R, Martin P, Le Lay S
Journal of extracellular vesicles 2017;6(1):1305677
Journal of extracellular vesicles 2017;6(1):1305677
Distinct repertoires of microRNAs present in mouse astrocytes compared to astrocyte-secreted exosomes.
Jovičić A, Gitler AD
PloS one 2017;12(2):e0171418
PloS one 2017;12(2):e0171418
Extracellular vesicles mediate signaling between the aqueous humor producing and draining cells in the ocular system.
Lerner N, Avissar S, Beit-Yannai E
PloS one 2017;12(2):e0171153
PloS one 2017;12(2):e0171153
Radiation alters the cargo of exosomes released from squamous head and neck cancer cells to promote migration of recipient cells.
Mutschelknaus L, Azimzadeh O, Heider T, Winkler K, Vetter M, Kell R, Tapio S, Merl-Pham J, Huber SM, Edalat L, Radulović V, Anastasov N, Atkinson MJ, Moertl S
Scientific reports 2017 Sep 29;7(1):12423
Scientific reports 2017 Sep 29;7(1):12423
Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA.
Köster S, Upadhyay S, Chandra P, Papavinasasundaram K, Yang G, Hassan A, Grigsby SJ, Mittal E, Park HS, Jones V, Hsu FF, Jackson M, Sassetti CM, Philips JA
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 10;114(41):E8711-E8720
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 10;114(41):E8711-E8720
Tsg101 chaperone function revealed by HIV-1 assembly inhibitors.
Strickland M, Ehrlich LS, Watanabe S, Khan M, Strub MP, Luan CH, Powell MD, Leis J, Tjandra N, Carter CA
Nature communications 2017 Nov 9;8(1):1391
Nature communications 2017 Nov 9;8(1):1391
Exosome secretion promotes chemotaxis of cancer cells.
Sung BH, Weaver AM
Cell adhesion & migration 2017 Mar 4;11(2):187-195
Cell adhesion & migration 2017 Mar 4;11(2):187-195
Reciprocal regulation of γ-globin expression by exo-miRNAs: Relevance to γ-globin silencing in β-thalassemia major.
Sun KT, Huang YN, Palanisamy K, Chang SS, Wang IK, Wu KH, Chen P, Peng CT, Li CY
Scientific reports 2017 Mar 16;7(1):202
Scientific reports 2017 Mar 16;7(1):202
Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation.
Yentrapalli R, Merl-Pham J, Azimzadeh O, Mutschelknaus L, Peters C, Hauck SM, Atkinson MJ, Tapio S, Moertl S
International journal of radiation biology 2017 Jun;93(6):569-580
International journal of radiation biology 2017 Jun;93(6):569-580
Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy.
Guo H, Chitiprolu M, Roncevic L, Javalet C, Hemming FJ, Trung MT, Meng L, Latreille E, Tanese de Souza C, McCulloch D, Baldwin RM, Auer R, Côté J, Russell RC, Sadoul R, Gibbings D
Developmental cell 2017 Dec 18;43(6):716-730.e7
Developmental cell 2017 Dec 18;43(6):716-730.e7
Enhanced exosome secretion in Down syndrome brain - a protective mechanism to alleviate neuronal endosomal abnormalities.
Gauthier SA, Pérez-González R, Sharma A, Huang FK, Alldred MJ, Pawlik M, Kaur G, Ginsberg SD, Neubert TA, Levy E
Acta neuropathologica communications 2017 Aug 29;5(1):65
Acta neuropathologica communications 2017 Aug 29;5(1):65
Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A.
Willén K, Edgar JR, Hasegawa T, Tanaka N, Futter CE, Gouras GK
Molecular neurodegeneration 2017 Aug 23;12(1):61
Molecular neurodegeneration 2017 Aug 23;12(1):61
Tetherin is an exosomal tether.
Edgar JR, Manna PT, Nishimura S, Banting G, Robinson MS
eLife 2016 Sep 22;5
eLife 2016 Sep 22;5
Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS.
Kamelgarn M, Chen J, Kuang L, Arenas A, Zhai J, Zhu H, Gal J
Biochimica et biophysica acta 2016 Oct;1862(10):2004-14
Biochimica et biophysica acta 2016 Oct;1862(10):2004-14
ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.
Villarroya-Beltri C, Baixauli F, Mittelbrunn M, Fernández-Delgado I, Torralba D, Moreno-Gonzalo O, Baldanta S, Enrich C, Guerra S, Sánchez-Madrid F
Nature communications 2016 Nov 24;7:13588
Nature communications 2016 Nov 24;7:13588
Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway.
Park A, Yun T, Vigant F, Pernet O, Won ST, Dawes BE, Bartkowski W, Freiberg AN, Lee B
PLoS pathogens 2016 May;12(5):e1005659
PLoS pathogens 2016 May;12(5):e1005659
Cortactin promotes exosome secretion by controlling branched actin dynamics.
Sinha S, Hoshino D, Hong NH, Kirkbride KC, Grega-Larson NE, Seiki M, Tyska MJ, Weaver AM
The Journal of cell biology 2016 Jul 18;214(2):197-213
The Journal of cell biology 2016 Jul 18;214(2):197-213
The Endocytic Fate of the Transferrin Receptor Is Regulated by c-Abl Kinase.
Cao H, Schroeder B, Chen J, Schott MB, McNiven MA
The Journal of biological chemistry 2016 Aug 5;291(32):16424-37
The Journal of biological chemistry 2016 Aug 5;291(32):16424-37
Y-box protein 1 is required to sort microRNAs into exosomes in cells and in a cell-free reaction.
Shurtleff MJ, Temoche-Diaz MM, Karfilis KV, Ri S, Schekman R
eLife 2016 Aug 25;5
eLife 2016 Aug 25;5
Physical exercise induces rapid release of small extracellular vesicles into the circulation.
Frühbeis C, Helmig S, Tug S, Simon P, Krämer-Albers EM
Journal of extracellular vesicles 2015;4:28239
Journal of extracellular vesicles 2015;4:28239
RAL-1 controls multivesicular body biogenesis and exosome secretion.
Hyenne V, Apaydin A, Rodriguez D, Spiegelhalter C, Hoff-Yoessle S, Diem M, Tak S, Lefebvre O, Schwab Y, Goetz JG, Labouesse M
The Journal of cell biology 2015 Oct 12;211(1):27-37
The Journal of cell biology 2015 Oct 12;211(1):27-37
Cell-to-cell propagation of the bacterial toxin CNF1 via extracellular vesicles: potential impact on the therapeutic use of the toxin.
Fabbri A, Cori S, Zanetti C, Guidotti M, Sargiacomo M, Loizzo S, Fiorentini C
Toxins 2015 Nov 5;7(11):4610-21
Toxins 2015 Nov 5;7(11):4610-21
Translocation of interleukin-1β into a vesicle intermediate in autophagy-mediated secretion.
Zhang M, Kenny SJ, Ge L, Xu K, Schekman R
eLife 2015 Nov 2;4
eLife 2015 Nov 2;4
Extracellular vesicle sorting of α-Synuclein is regulated by sumoylation.
Kunadt M, Eckermann K, Stuendl A, Gong J, Russo B, Strauss K, Rai S, Kügler S, Falomir Lockhart L, Schwalbe M, Krumova P, Oliveira LM, Bähr M, Möbius W, Levin J, Giese A, Kruse N, Mollenhauer B, Geiss-Friedlander R, Ludolph AC, Freischmidt A, Feiler MS, Danzer KM, Zweckstetter M, Jovin TM, Simons M, Weishaupt JH, Schneider A
Acta neuropathologica 2015 May;129(5):695-713
Acta neuropathologica 2015 May;129(5):695-713
Exosomes released by keratinocytes modulate melanocyte pigmentation.
Lo Cicero A, Delevoye C, Gilles-Marsens F, Loew D, Dingli F, Guéré C, André N, Vié K, van Niel G, Raposo G
Nature communications 2015 Jun 24;6:7506
Nature communications 2015 Jun 24;6:7506
WASH and Tsg101/ALIX-dependent diversion of stress-internalized EGFR from the canonical endocytic pathway.
Tomas A, Vaughan SO, Burgoyne T, Sorkin A, Hartley JA, Hochhauser D, Futter CE
Nature communications 2015 Jun 12;6:7324
Nature communications 2015 Jun 12;6:7324
ESCRTs regulate amyloid precursor protein sorting in multivesicular bodies and intracellular amyloid-β accumulation.
Edgar JR, Willén K, Gouras GK, Futter CE
Journal of cell science 2015 Jul 15;128(14):2520-8
Journal of cell science 2015 Jul 15;128(14):2520-8
Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites.
Prescher J, Baumgärtel V, Ivanchenko S, Torrano AA, Bräuchle C, Müller B, Lamb DC
PLoS pathogens 2015 Feb;11(2):e1004677
PLoS pathogens 2015 Feb;11(2):e1004677
Spatial control of Shoc2-scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5.
Jang ER, Jang H, Shi P, Popa G, Jeoung M, Galperin E
Journal of cell science 2015 Dec 1;128(23):4428-41
Journal of cell science 2015 Dec 1;128(23):4428-41
Functional Interaction Between the ESCRT-I Component TSG101 and the HSV-1 Tegument Ubiquitin Specific Protease.
Calistri A, Munegato D, Toffoletto M, Celestino M, Franchin E, Comin A, Sartori E, Salata C, Parolin C, Palù G
Journal of cellular physiology 2015 Aug;230(8):1794-806
Journal of cellular physiology 2015 Aug;230(8):1794-806
Vascular smooth muscle cell calcification is mediated by regulated exosome secretion.
Kapustin AN, Chatrou ML, Drozdov I, Zheng Y, Davidson SM, Soong D, Furmanik M, Sanchis P, De Rosales RT, Alvarez-Hernandez D, Shroff R, Yin X, Muller K, Skepper JN, Mayr M, Reutelingsperger CP, Chester A, Bertazzo S, Schurgers LJ, Shanahan CM
Circulation research 2015 Apr 10;116(8):1312-23
Circulation research 2015 Apr 10;116(8):1312-23
The multivesicular body is the major internal site of prion conversion.
Yim YI, Park BC, Yadavalli R, Zhao X, Eisenberg E, Greene LE
Journal of cell science 2015 Apr 1;128(7):1434-43
Journal of cell science 2015 Apr 1;128(7):1434-43
Septin6 and Septin7 GTP binding proteins regulate AP-3- and ESCRT-dependent multivesicular body biogenesis.
Traikov S, Stange C, Wassmer T, Paul-Gilloteaux P, Salamero J, Raposo G, Hoflack B
PloS one 2014;9(11):e109372
PloS one 2014;9(11):e109372
ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation.
Kojima K, Amano Y, Yoshino K, Tanaka N, Sugamura K, Takeshita T
The Journal of biological chemistry 2014 Nov 28;289(48):33296-310
The Journal of biological chemistry 2014 Nov 28;289(48):33296-310
ATP13A2/PARK9 regulates secretion of exosomes and α-synuclein.
Tsunemi T, Hamada K, Krainc D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Nov 12;34(46):15281-7
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Nov 12;34(46):15281-7
Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer's disease.
Armstrong A, Mattsson N, Appelqvist H, Janefjord C, Sandin L, Agholme L, Olsson B, Svensson S, Blennow K, Zetterberg H, Kågedal K
Neuromolecular medicine 2014 Mar;16(1):150-60
Neuromolecular medicine 2014 Mar;16(1):150-60
ESCRT regulates surface expression of the Kir2.1 potassium channel.
Kolb AR, Needham PG, Rothenberg C, Guerriero CJ, Welling PA, Brodsky JL
Molecular biology of the cell 2014 Jan;25(2):276-89
Molecular biology of the cell 2014 Jan;25(2):276-89
Functional significance of macrophage-derived exosomes in inflammation and pain.
McDonald MK, Tian Y, Qureshi RA, Gormley M, Ertel A, Gao R, Aradillas Lopez E, Alexander GM, Sacan A, Fortina P, Ajit SK
Pain 2014 Aug;155(8):1527-39
Pain 2014 Aug;155(8):1527-39
NDRG1 functions in LDL receptor trafficking by regulating endosomal recycling and degradation.
Pietiäinen V, Vassilev B, Blom T, Wang W, Nelson J, Bittman R, Bäck N, Zelcer N, Ikonen E
Journal of cell science 2013 Sep 1;126(Pt 17):3961-71
Journal of cell science 2013 Sep 1;126(Pt 17):3961-71
Identification of late assembly domains of the human endogenous retrovirus-K(HML-2).
Chudak C, Beimforde N, George M, Zimmermann A, Lausch V, Hanke K, Bannert N
Retrovirology 2013 Nov 19;10:140
Retrovirology 2013 Nov 19;10:140
Macrophage-derived matrix vesicles: an alternative novel mechanism for microcalcification in atherosclerotic plaques.
New SE, Goettsch C, Aikawa M, Marchini JF, Shibasaki M, Yabusaki K, Libby P, Shanahan CM, Croce K, Aikawa E
Circulation research 2013 Jun 21;113(1):72-7
Circulation research 2013 Jun 21;113(1):72-7
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.
Frühbeis C, Fröhlich D, Kuo WP, Amphornrat J, Thilemann S, Saab AS, Kirchhoff F, Möbius W, Goebbels S, Nave KA, Schneider A, Simons M, Klugmann M, Trotter J, Krämer-Albers EM
PLoS biology 2013 Jul;11(7):e1001604
PLoS biology 2013 Jul;11(7):e1001604
Tsg101 interacts with herpes simplex virus 1 VP1/2 and is a substrate of VP1/2 ubiquitin-specific protease domain activity.
Caduco M, Comin A, Toffoletto M, Munegato D, Sartori E, Celestino M, Salata C, Parolin C, Palù G, Calistri A
Journal of virology 2013 Jan;87(1):692-6
Journal of virology 2013 Jan;87(1):692-6
RAB7 and TSG101 are required for the constitutive recycling of unliganded EGFRs via distinct mechanisms.
Rush JS, Ceresa BP
Molecular and cellular endocrinology 2013 Dec 5;381(1-2):188-97
Molecular and cellular endocrinology 2013 Dec 5;381(1-2):188-97
Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles.
Colombo M, Moita C, van Niel G, Kowal J, Vigneron J, Benaroch P, Manel N, Moita LF, Théry C, Raposo G
Journal of cell science 2013 Dec 15;126(Pt 24):5553-65
Journal of cell science 2013 Dec 15;126(Pt 24):5553-65
The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation.
Sorrentino V, Nelson JK, Maspero E, Marques AR, Scheer L, Polo S, Zelcer N
Journal of lipid research 2013 Aug;54(8):2174-84
Journal of lipid research 2013 Aug;54(8):2174-84
Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation.
Bobrie A, Colombo M, Krumeich S, Raposo G, Théry C
Journal of extracellular vesicles 2012;1
Journal of extracellular vesicles 2012;1
The ESCRT machinery is recruited by the viral BFRF1 protein to the nucleus-associated membrane for the maturation of Epstein-Barr Virus.
Lee CP, Liu PT, Kung HN, Su MT, Chua HH, Chang YH, Chang CW, Tsai CH, Liu FT, Chen MR
PLoS pathogens 2012 Sep;8(9):e1002904
PLoS pathogens 2012 Sep;8(9):e1002904
Regulation of the EGF transcriptional response by endocytic sorting.
Brankatschk B, Wichert SP, Johnson SD, Schaad O, Rossner MJ, Gruenberg J
Science signaling 2012 Mar 13;5(215):ra21
Science signaling 2012 Mar 13;5(215):ra21
Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.
Nabhan JF, Hu R, Oh RS, Cohen SN, Lu Q
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 13;109(11):4146-51
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 13;109(11):4146-51
Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid β (Aβ) production in the trans-Golgi network.
Choy RW, Cheng Z, Schekman R
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):E2077-82
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):E2077-82
Regulation of the MVB pathway by SCAMP3.
Falguières T, Castle D, Gruenberg J
Traffic (Copenhagen, Denmark) 2012 Jan;13(1):131-42
Traffic (Copenhagen, Denmark) 2012 Jan;13(1):131-42
Endosomal accumulation of the activated epidermal growth factor receptor (EGFR) induces apoptosis.
Rush JS, Quinalty LM, Engelman L, Sherry DM, Ceresa BP
The Journal of biological chemistry 2012 Jan 2;287(1):712-22
The Journal of biological chemistry 2012 Jan 2;287(1):712-22
Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport.
Pasqual G, Rojek JM, Masin M, Chatton JY, Kunz S
PLoS pathogens 2011 Sep;7(9):e1002232
PLoS pathogens 2011 Sep;7(9):e1002232
MARCH ubiquitin ligases alter the itinerary of clathrin-independent cargo from recycling to degradation.
Eyster CA, Cole NB, Petersen S, Viswanathan K, Früh K, Donaldson JG
Molecular biology of the cell 2011 Sep;22(17):3218-30
Molecular biology of the cell 2011 Sep;22(17):3218-30
Impaired β-amyloid secretion in Alzheimer's disease pathogenesis.
Tampellini D, Rahman N, Lin MT, Capetillo-Zarate E, Gouras GK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Oct 26;31(43):15384-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Oct 26;31(43):15384-90
The PTAP sequence within the p6 domain of human immunodeficiency virus type 1 Gag regulates its ubiquitination and MHC class I antigen presentation.
Hahn S, Setz C, Wild J, Schubert U
Journal of immunology (Baltimore, Md. : 1950) 2011 May 15;186(10):5706-18
Journal of immunology (Baltimore, Md. : 1950) 2011 May 15;186(10):5706-18
ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes.
Balut CM, Gao Y, Murray SA, Thibodeau PH, Devor DC
American journal of physiology. Cell physiology 2010 Nov;299(5):C1015-27
American journal of physiology. Cell physiology 2010 Nov;299(5):C1015-27
Quality control for unfolded proteins at the plasma membrane.
Apaja PM, Xu H, Lukacs GL
The Journal of cell biology 2010 Nov 1;191(3):553-70
The Journal of cell biology 2010 Nov 1;191(3):553-70
A targeted spatial-temporal proteomics approach implicates multiple cellular trafficking pathways in human cytomegalovirus virion maturation.
Moorman NJ, Sharon-Friling R, Shenk T, Cristea IM
Molecular & cellular proteomics : MCP 2010 May;9(5):851-60
Molecular & cellular proteomics : MCP 2010 May;9(5):851-60
c-IAP1 and UbcH5 promote K11-linked polyubiquitination of RIP1 in TNF signalling.
Dynek JN, Goncharov T, Dueber EC, Fedorova AV, Izrael-Tomasevic A, Phu L, Helgason E, Fairbrother WJ, Deshayes K, Kirkpatrick DS, Vucic D
The EMBO journal 2010 Dec 15;29(24):4198-209
The EMBO journal 2010 Dec 15;29(24):4198-209
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
Inuzuka T, Suzuki H, Kawasaki M, Shibata H, Wakatsuki S, Maki M
BMC structural biology 2010 Aug 6;10:25
BMC structural biology 2010 Aug 6;10:25
ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane.
Truschel ST, Simoes S, Setty SR, Harper DC, Tenza D, Thomas PC, Herman KE, Sackett SD, Cowan DC, Theos AC, Raposo G, Marks MS
Traffic (Copenhagen, Denmark) 2009 Sep;10(9):1318-36
Traffic (Copenhagen, Denmark) 2009 Sep;10(9):1318-36
Transferrin-directed internalization and cycling of transferrin receptor 2.
Chen J, Wang J, Meyers KR, Enns CA
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1488-501
Traffic (Copenhagen, Denmark) 2009 Oct;10(10):1488-501
Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.
Leithe E, Kjenseth A, Sirnes S, Stenmark H, Brech A, Rivedal E
Journal of cell science 2009 Nov 1;122(Pt 21):3883-93
Journal of cell science 2009 Nov 1;122(Pt 21):3883-93
SCAMP3 negatively regulates epidermal growth factor receptor degradation and promotes receptor recycling.
Aoh QL, Castle AM, Hubbard CH, Katsumata O, Castle JD
Molecular biology of the cell 2009 Mar;20(6):1816-32
Molecular biology of the cell 2009 Mar;20(6):1816-32
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
Glozman R, Okiyoneda T, Mulvihill CM, Rini JM, Barriere H, Lukacs GL
The Journal of cell biology 2009 Mar 23;184(6):847-62
The Journal of cell biology 2009 Mar 23;184(6):847-62
Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
Okumura M, Ichioka F, Kobayashi R, Suzuki H, Yoshida H, Shibata H, Maki M
Biochemical and biophysical research communications 2009 Aug 14;386(1):237-41
Biochemical and biophysical research communications 2009 Aug 14;386(1):237-41
Hrs and SNX3 functions in sorting and membrane invagination within multivesicular bodies.
Pons V, Luyet PP, Morel E, Abrami L, van der Goot FG, Parton RG, Gruenberg J
PLoS biology 2008 Sep 2;6(9):e214
PLoS biology 2008 Sep 2;6(9):e214
In vitro budding of intralumenal vesicles into late endosomes is regulated by Alix and Tsg101.
Falguières T, Luyet PP, Bissig C, Scott CC, Velluz MC, Gruenberg J
Molecular biology of the cell 2008 Nov;19(11):4942-55
Molecular biology of the cell 2008 Nov;19(11):4942-55
Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4).
Ichioka F, Kobayashi R, Katoh K, Shibata H, Maki M
The FEBS journal 2008 Feb;275(4):682-92
The FEBS journal 2008 Feb;275(4):682-92
Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag.
Valiathan RR, Resh MD
Virology journal 2008 Feb 11;5:23
Virology journal 2008 Feb 11;5:23
The ESCRT-I subunit TSG101 controls endosome-to-cytosol release of viral RNA.
Luyet PP, Falguières T, Pons V, Pattnaik AK, Gruenberg J
Traffic (Copenhagen, Denmark) 2008 Dec;9(12):2279-90
Traffic (Copenhagen, Denmark) 2008 Dec;9(12):2279-90
Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.
Shibata H, Suzuki H, Kakiuchi T, Inuzuka T, Yoshida H, Mizuno T, Maki M
The Journal of biological chemistry 2008 Apr 11;283(15):9623-32
The Journal of biological chemistry 2008 Apr 11;283(15):9623-32
MVB-12, a fourth subunit of metazoan ESCRT-I, functions in receptor downregulation.
Audhya A, McLeod IX, Yates JR, Oegema K
PloS one 2007 Sep 26;2(9):e956
PloS one 2007 Sep 26;2(9):e956
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Krämer-Albers EM, Bretz N, Tenzer S, Winterstein C, Möbius W, Berger H, Nave KA, Schild H, Trotter J
Proteomics. Clinical applications 2007 Nov;1(11):1446-61
Proteomics. Clinical applications 2007 Nov;1(11):1446-61
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease.
Filimonenko M, Stuffers S, Raiborg C, Yamamoto A, Malerød L, Fisher EM, Isaacs A, Brech A, Stenmark H, Simonsen A
The Journal of cell biology 2007 Nov 5;179(3):485-500
The Journal of cell biology 2007 Nov 5;179(3):485-500
Internalized antibodies to the Abeta domain of APP reduce neuronal Abeta and protect against synaptic alterations.
Tampellini D, Magrané J, Takahashi RH, Li F, Lin MT, Almeida CG, Gouras GK
The Journal of biological chemistry 2007 Jun 29;282(26):18895-906
The Journal of biological chemistry 2007 Jun 29;282(26):18895-906
The molecular mechanism of hepcidin-mediated ferroportin down-regulation.
De Domenico I, Ward DM, Langelier C, Vaughn MB, Nemeth E, Sundquist WI, Ganz T, Musci G, Kaplan J
Molecular biology of the cell 2007 Jul;18(7):2569-78
Molecular biology of the cell 2007 Jul;18(7):2569-78
The ESCRT machinery is not required for human cytomegalovirus envelopment.
Fraile-Ramos A, Pelchen-Matthews A, Risco C, Rejas MT, Emery VC, Hassan-Walker AF, Esteban M, Marsh M
Cellular microbiology 2007 Dec;9(12):2955-67
Cellular microbiology 2007 Dec;9(12):2955-67
Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages.
Chertova E, Chertov O, Coren LV, Roser JD, Trubey CM, Bess JW Jr, Sowder RC 2nd, Barsov E, Hood BL, Fisher RJ, Nagashima K, Conrads TP, Veenstra TD, Lifson JD, Ott DE
Journal of virology 2006 Sep;80(18):9039-52
Journal of virology 2006 Sep;80(18):9039-52
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
Langelier C, von Schwedler UK, Fisher RD, De Domenico I, White PL, Hill CP, Kaplan J, Ward D, Sundquist WI
Journal of virology 2006 Oct;80(19):9465-80
Journal of virology 2006 Oct;80(19):9465-80
YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42.
Hui EK, Barman S, Tang DH, France B, Nayak DP
Journal of virology 2006 Mar;80(5):2291-308
Journal of virology 2006 Mar;80(5):2291-308
An essential role for SNX1 in lysosomal sorting of protease-activated receptor-1: evidence for retromer-, Hrs-, and Tsg101-independent functions of sorting nexins.
Gullapalli A, Wolfe BL, Griffin CT, Magnuson T, Trejo J
Molecular biology of the cell 2006 Mar;17(3):1228-38
Molecular biology of the cell 2006 Mar;17(3):1228-38
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
Bache KG, Stuffers S, Malerød L, Slagsvold T, Raiborg C, Lechardeur D, Wälchli S, Lukacs GL, Brech A, Stenmark H
Molecular biology of the cell 2006 Jun;17(6):2513-23
Molecular biology of the cell 2006 Jun;17(6):2513-23
Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains.
Raiborg C, Wesche J, Malerød L, Stenmark H
Journal of cell science 2006 Jun 15;119(Pt 12):2414-24
Journal of cell science 2006 Jun 15;119(Pt 12):2414-24
Distinct roles for Tsg101 and Hrs in multivesicular body formation and inward vesiculation.
Razi M, Futter CE
Molecular biology of the cell 2006 Aug;17(8):3469-83
Molecular biology of the cell 2006 Aug;17(8):3469-83
Beta-amyloid accumulation impairs multivesicular body sorting by inhibiting the ubiquitin-proteasome system.
Almeida CG, Takahashi RH, Gouras GK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 19;26(16):4277-88
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 19;26(16):4277-88
Lysine-63-linked ubiquitination is required for endolysosomal degradation of class I molecules.
Duncan LM, Piper S, Dodd RB, Saville MK, Sanderson CM, Luzio JP, Lehner PJ
The EMBO journal 2006 Apr 19;25(8):1635-45
The EMBO journal 2006 Apr 19;25(8):1635-45
L-domain flanking sequences are important for host interactions and efficient budding of vesicular stomatitis virus recombinants.
Irie T, Harty RN
Journal of virology 2005 Oct;79(20):12617-22
Journal of virology 2005 Oct;79(20):12617-22
The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B.
Katoh K, Suzuki H, Terasawa Y, Mizuno T, Yasuda J, Shibata H, Maki M
The Biochemical journal 2005 Nov 1;391(Pt 3):677-85
The Biochemical journal 2005 Nov 1;391(Pt 3):677-85
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J
The Journal of biological chemistry 2005 Mar 18;280(11):10548-55
The Journal of biological chemistry 2005 Mar 18;280(11):10548-55
Functional characterization of Ebola virus L-domains using VSV recombinants.
Irie T, Licata JM, Harty RN
Virology 2005 Jun 5;336(2):291-8
Virology 2005 Jun 5;336(2):291-8
Alix regulates cortical actin and the spatial distribution of endosomes.
Cabezas A, Bache KG, Brech A, Stenmark H
Journal of cell science 2005 Jun 15;118(Pt 12):2625-35
Journal of cell science 2005 Jun 15;118(Pt 12):2625-35
Interaction of the mammalian endosomal sorting complex required for transport (ESCRT) III protein hSnf7-1 with itself, membranes, and the AAA+ ATPase SKD1.
Lin Y, Kimpler LA, Naismith TV, Lauer JM, Hanson PI
The Journal of biological chemistry 2005 Apr 1;280(13):12799-809
The Journal of biological chemistry 2005 Apr 1;280(13):12799-809
The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation.
Bache KG, Slagsvold T, Cabezas A, Rosendal KR, Raiborg C, Stenmark H
Molecular biology of the cell 2004 Sep;15(9):4337-46
Molecular biology of the cell 2004 Sep;15(9):4337-46
Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A.
Irie T, Licata JM, McGettigan JP, Schnell MJ, Harty RN
Journal of virology 2004 Mar;78(6):2657-65
Journal of virology 2004 Mar;78(6):2657-65
Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins.
Heissmeyer V, Macián F, Im SH, Varma R, Feske S, Venuprasad K, Gu H, Liu YC, Dustin ML, Rao A
Nature immunology 2004 Mar;5(3):255-65
Nature immunology 2004 Mar;5(3):255-65
Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes.
Sharma M, Pampinella F, Nemes C, Benharouga M, So J, Du K, Bache KG, Papsin B, Zerangue N, Stenmark H, Lukacs GL
The Journal of cell biology 2004 Mar 15;164(6):923-33
The Journal of cell biology 2004 Mar 15;164(6):923-33
The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist WI
The Journal of biological chemistry 2004 Aug 20;279(34):36059-71
The Journal of biological chemistry 2004 Aug 20;279(34):36059-71
Reduction of TSG101 protein has a negative impact on tumor cell growth.
Zhu G, Gilchrist R, Borley N, Chng HW, Morgan M, Marshall JF, Camplejohn RS, Muir GH, Hart IR
International journal of cancer 2004 Apr 20;109(4):541-7
International journal of cancer 2004 Apr 20;109(4):541-7
The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release.
Gottwein E, Bodem J, Müller B, Schmechel A, Zentgraf H, Kräusslich HG
Journal of virology 2003 Sep;77(17):9474-85
Journal of virology 2003 Sep;77(17):9474-85
The protein network of HIV budding.
von Schwedler UK, Stuchell M, Müller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, Scott A, Kräusslich HG, Kaplan J, Morham SG, Sundquist WI
Cell 2003 Sep 19;114(6):701-13
Cell 2003 Sep 19;114(6):701-13
AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
Strack B, Calistri A, Craig S, Popova E, Göttlinger HG
Cell 2003 Sep 19;114(6):689-99
Cell 2003 Sep 19;114(6):689-99
Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein.
Chiou CT, Hu CC, Chen PH, Liao CL, Lin YL, Wang JJ
The Journal of general virology 2003 Oct;84(Pt 10):2795-805
The Journal of general virology 2003 Oct;84(Pt 10):2795-805
Combination of microdissection and microarray analysis to identify gene expression changes between differentially located tumour cells in breast cancer.
Zhu G, Reynolds L, Crnogorac-Jurcevic T, Gillett CE, Dublin EA, Marshall JF, Barnes D, D'Arrigo C, Van Trappen PO, Lemoine NR, Hart IR
Oncogene 2003 Jun 12;22(24):3742-8
Oncogene 2003 Jun 12;22(24):3742-8
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes.
Bache KG, Brech A, Mehlum A, Stenmark H
The Journal of cell biology 2003 Aug 4;162(3):435-42
The Journal of cell biology 2003 Aug 4;162(3):435-42
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.
Pornillos O, Higginson DS, Stray KM, Fisher RD, Garrus JE, Payne M, He GP, Wang HE, Morham SG, Sundquist WI
The Journal of cell biology 2003 Aug 4;162(3):425-34
The Journal of cell biology 2003 Aug 4;162(3):425-34
Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 gag polyprotein and results in increased levels of ubiquitinated gag.
Myers EL, Allen JF
Journal of virology 2002 Nov;76(22):11226-35
Journal of virology 2002 Nov;76(22):11226-35
Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
Bishop N, Horman A, Woodman P
The Journal of cell biology 2002 Apr 1;157(1):91-101
The Journal of cell biology 2002 Apr 1;157(1):91-101
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist WI
Cell 2001 Oct 5;107(1):55-65
Cell 2001 Oct 5;107(1):55-65
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Detection of human TSG101 protein in HeLa whole cell lysate using GeneTex TSG101 4A10 monoclonal antibody GTX70255.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 10% SDS PAGE GTX70255 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: C2C12 10% SDS PAGE GTX70255 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extract10% SDS-PAGETSG101 antibody [4A10] (GTX70255) dilution: 1:500The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extractB. 30 ?g JC whole cell lysate/extractC. 30 ?g BCL-1 whole cell lysate/extract10% SDS-PAGETSG101 antibody [4A10] (GTX70255) dilution: 1:500The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 ?g/mL and GTX70255 for detection at a concentration of 1.5 ?g/mL.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using TSG101(GTX70255) antibody at 1:100 dilution.
