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- Antibody Data
- Antigen structure
- References [16]
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- Western blot [1]
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- Product number
- HPA006161 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA006161, RRID:AB_1080408
- Product name
- Anti-TSG101
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TSG101, Gene description: tumor susceptibility 101, Alternative Gene Names: TSG10, VPS23, Validated applications: ICC, IHC, WB, Uniprot ID: Q99816, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Profiling Small RNA From Brain Extracellular Vesicles in Individuals With Depression
Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina.
Comparison of methods of isolating extracellular vesicle microRNA from HepG2 cells for High-throughput sequencing
Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell–Cell Communication in Cancer
Identification and Validation of miR-222-3p and miR-409-3p as Plasma Biomarkers in Gestational Diabetes Mellitus Sharing Validated Target Genes Involved in Metabolic Homeostasis.
Small nucleolar RNAs in plasma extracellular vesicles and their discriminatory power as diagnostic biomarkers of Alzheimer's disease
Modulation of Early Neutrophil Granulation: The Circulating Tumor Cell-Extravesicular Connection in Pancreatic Ductal Adenocarcinoma
RAB31 marks and controls an ESCRT-independent exosome pathway
Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium
Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity
Feasibility of urinary extracellular vesicle proteome profiling using a robust and simple, clinically applicable isolation method
A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.
Modulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer
Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells
Active Wnt proteins are secreted on exosomes
Ibrahim P, Denniston R, Mitsuhashi H, Yang J, Fiori L, Żurawek D, Mechawar N, Nagy C, Turecki G
International Journal of Neuropsychopharmacology 2024;27(3)
International Journal of Neuropsychopharmacology 2024;27(3)
Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina.
Touahri Y, Hanna J, Tachibana N, Okawa S, Liu H, David LA, Olender T, Vasan L, Pak A, Mehta DN, Chinchalongporn V, Balakrishnan A, Cantrup R, Dixit R, Mattar P, Saleh F, Ilnytskyy Y, Murshed M, Mains PE, Kovalchuk I, Lefebvre JL, Leong HS, Cayouette M, Wang C, Del Sol A, Brand M, Reese BE, Schuurmans C
Cell reports 2024 Apr 23;43(4):114005
Cell reports 2024 Apr 23;43(4):114005
Comparison of methods of isolating extracellular vesicle microRNA from HepG2 cells for High-throughput sequencing
Xiong Y, Fan X, Chen Y, Huang Y, Quan J, Yi P, Xiao M, Huang Z, Hu X
Frontiers in Molecular Biosciences 2022;9
Frontiers in Molecular Biosciences 2022;9
Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell–Cell Communication in Cancer
Chetty V, Ghanam J, Anchan S, Reinhardt K, Brenzel A, Gelléri M, Cremer C, Grueso-Navarro E, Schneider M, von Neuhoff N, Reinhardt D, Jablonska J, Nazarenko I, Thakur B
Cancers 2022;14(9):2068
Cancers 2022;14(9):2068
Identification and Validation of miR-222-3p and miR-409-3p as Plasma Biomarkers in Gestational Diabetes Mellitus Sharing Validated Target Genes Involved in Metabolic Homeostasis.
Filardi T, Catanzaro G, Grieco GE, Splendiani E, Trocchianesi S, Santangelo C, Brunelli R, Guarino E, Sebastiani G, Dotta F, Morano S, Ferretti E
International journal of molecular sciences 2022 Apr 12;23(8)
International journal of molecular sciences 2022 Apr 12;23(8)
Small nucleolar RNAs in plasma extracellular vesicles and their discriminatory power as diagnostic biomarkers of Alzheimer's disease
Fitz N, Wang J, Kamboh M, Koldamova R, Lefterov I
Neurobiology of Disease 2021;159
Neurobiology of Disease 2021;159
Modulation of Early Neutrophil Granulation: The Circulating Tumor Cell-Extravesicular Connection in Pancreatic Ductal Adenocarcinoma
Charles Jacob H, Charles Richard J, Signorelli R, Kashuv T, Lavania S, Vaish U, Boopathy R, Middleton A, Boone M, Sundaram R, Dudeja V, Saluja A
Cancers 2021;13(11):2727
Cancers 2021;13(11):2727
RAB31 marks and controls an ESCRT-independent exosome pathway
Wei D, Zhan W, Gao Y, Huang L, Gong R, Wang W, Zhang R, Wu Y, Gao S, Kang T
Cell Research 2020;31(2):157-177
Cell Research 2020;31(2):157-177
Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium
Linnemannstöns K, Witte L, Karuna M P, Kittel J, Danieli A, Müller D, Nitsch L, Honemann-Capito M, Grawe F, Wodarz A, Gross J
Development 2020;147(15)
Development 2020;147(15)
Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity
Karuna M P, Witte L, Linnemannstoens K, Choezom D, Danieli-Mackay A, Honemann-Capito M, Gross J
Biomolecules 2020;10(11):1560
Biomolecules 2020;10(11):1560
Feasibility of urinary extracellular vesicle proteome profiling using a robust and simple, clinically applicable isolation method
Bijnsdorp I, Maxouri O, Kardar A, Schelfhorst T, Piersma S, Pham T, Vis A, van Moorselaar R, Jimenez C
Journal of Extracellular Vesicles 2017;6(1)
Journal of Extracellular Vesicles 2017;6(1)
A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.
Imbert PR, Louche A, Luizet JB, Grandjean T, Bigot S, Wood TE, Gagné S, Blanco A, Wunderley L, Terradot L, Woodman P, Garvis S, Filloux A, Guery B, Salcedo SP
The EMBO journal 2017 Jul 3;36(13):1869-1887
The EMBO journal 2017 Jul 3;36(13):1869-1887
Modulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer
Soekmadji C, Riches J, Russell P, Ruelcke J, McPherson S, Wang C, Hovens C, Corcoran N, Hill M, Nelson C
Oncotarget 2016;8(32):52237-52255
Oncotarget 2016;8(32):52237-52255
Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer
Pospichalova V, Svoboda J, Dave Z, Kotrbova A, Kaiser K, Klemova D, Ilkovics L, Hampl A, Crha I, Jandakova E, Minar L, Weinberger V, Bryja V
Journal of Extracellular Vesicles 2015;4(1)
Journal of Extracellular Vesicles 2015;4(1)
Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells
Gildea J, Seaton J, Victor K, Reyes C, Bigler Wang D, Pettigrew A, Courtner C, Shah N, Tran H, Van Sciver R, Carlson J, Felder R
Clinical Biochemistry 2014;47(15):89-94
Clinical Biochemistry 2014;47(15):89-94
Active Wnt proteins are secreted on exosomes
Gross J, Chaudhary V, Bartscherer K, Boutros M
Nature Cell Biology 2012;14(10):1036-1045
Nature Cell Biology 2012;14(10):1036-1045
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
