MA5-57935

antibody from Invitrogen Antibodies

Targeting: EFTUD2 SNRNP116, Snrp116, Snu114, U5-116KD

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-57935

Provider

Invitrogen Antibodies

Product name

EFTUD2 Recombinant Rabbit Monoclonal Antibody (2Z1K3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPLT EPIIKPVKTK KFTLMEQTLP VTVYEMDFLA DLMDNSELIR NVTLCGHLHH GKTCFVDCLI EQTHPEIRKR YDQDLCYTDI LFTEQERGVG IKSTPVTVVL PDTKGKSYLF NIMDT

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

2Z1K3

Vial size

20 µL

Concentration

1.1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of EFTUD2 using PC-12 cells. The cells were incubated using 1:400 dilution with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) (100x lens). Followed by further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) using a 1:500 dilution (Red). DAPI was used for nuclear staining (Blue). Then were counterstained with α-Tubulin Mouse mAb using a 1:400 dilution followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) using a 1:500 dilution (Green). DAPI was used for nuclear staining (Blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of EFTUD2 using HeLa cells. The cells were incubated using 1:400 dilution with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) (100x lens). Followed by further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) using a 1:500 dilution (Red). DAPI was used for nuclear staining (Blue). Then were counterstained with α-Tubulin Mouse mAb using a 1:400 dilution followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) using a 1:500 dilution (Green). DAPI was used for nuclear staining (Blue).

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of EFTUD2 from 500 µg C6 cells was performed using EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) at 2 µg. Rabbit IgG isotype control was used to precipitate the Control IgG sample. Western blot analysis of immunoprecipitates was conducted using Recombinant Monoclonal Antibody (Product # MA5-57935) at a dilution of 1:2, 000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 with paraffin-embedded Human breast cancer tissue. The tissue was incubated with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) using a 1:1500 dilution (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 with paraffin-embedded Human thyroid tissue. The tissue was incubated with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) using a 1:1500 dilution (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 with paraffin-embedded Human colon tissue. The tissue was incubated with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) using a 1:1500 dilution (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 with paraffin-embedded Mouse testis tissue. The tissue was incubated with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) using a 1:1500 dilution (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 with paraffin-embedded Rat colon tissue. The tissue was incubated with EFTUD2 Recombinant Monoclonal Antibody (Product # MA5-57936) using a 1:1500 dilution (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to staining.