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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB0329 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0329, RRID:AB_1019030
- Product name
- BAG2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant BAG2.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of the cytoplasmic quality control protein degradation pathway by BAG2.
Dai Q, Qian SB, Li HH, McDonough H, Borchers C, Huang D, Takayama S, Younger JM, Ren HY, Cyr DM, Patterson C
The Journal of biological chemistry 2005 Nov 18;280(46):38673-81
The Journal of biological chemistry 2005 Nov 18;280(46):38673-81
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BAG2. Using BAG2 polyclonal antibody (Cat # PAB0329) at 1 : 2000. HeLa stably transfected Myc-tagged CHIP (carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by western blot analysis of BAG2. This experiment was done to show the colocalization of CHIP and Bag2. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence confocal microscopy of BAG2. Using BAG2 polyclonal antibody (Cat # PAB0329) at 1 : 2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B) . Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.
- Validation comment
- Immunofluorescence
