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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-72897 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186.
Sun L, Chen G, Sun A, Wang Z, Huang H, Gao Z, Liang W, Liu C, Li K
Frontiers in oncology 2020;10:31
Frontiers in oncology 2020;10:31
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAG2 in HeLa stably transfected Myc-tagged CHIP (Carboxyl terminus of Hsp70-interacting protein). Samples were incubated in BAG2 polyclonal antibody (Product # PA5-72897). Immunocomplexes were resolved by SDS-PAGE followed by BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of BAG2 in HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Samples were incubated in BAG2 polyclonal antibody (Product # PA5-72897) using a dilution of 1:2000. Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of BAG2 in HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Samples were incubated in BAG2 polyclonal antibody (Product # PA5-72897) using a dilution of 1:2000. Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock.
