Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- PA5-30922 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (96%), Rat (95%), Rhesus Monkey (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references ARP-T1-associated Bazex-Dupré-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies.
BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186.
Park HS, Papanastasi E, Blanchard G, Chiticariu E, Bachmann D, Plomann M, Morice-Picard F, Vabres P, Smahi A, Huber M, Pich C, Hohl D
Communications biology 2021 May 10;4(1):544
Communications biology 2021 May 10;4(1):544
BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186.
Sun L, Chen G, Sun A, Wang Z, Huang H, Gao Z, Liang W, Liu C, Li K
Frontiers in oncology 2020;10:31
Frontiers in oncology 2020;10:31
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BAG2 Polyclonal Antibody (Product # PA5-30922). Sample (30 µg of whole cell lysate). Lane A: A549. 12% SDS PAGE. BAG2 Polyclonal Antibody (Product # PA5-30922) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BAG2 in methanol-fixed HeLa cells using a BAG2 polyclonal antibody (Product # PA5-30922) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using BAG2 (Product # PA5-30922) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 ARP-T1 interacts with proteins involved in ciliary machinery. a , b HeLa ( a ) and hTERT-RPE1 ( b ) cells were transduced with lentiviral vectors, empty vector (V), ACTRT1 mutant (M) and ACTRT1 WT (WT), and immunoprecipitated (IP) with anti-FLAG monoclonal antibody M2-conjugated agarose, and analyzed by immunoblot with indicated antisera. c Immunofluorescence stainings of ARP-T1, acetylated-tubulin and rootletin in 35 days of serum-starved ARPE19 cells. Nuclei are stained with DAPI. Scale bar, 5 um. Higher magnifications of the boxed area are shown on right three panels. Scale bar, 1 um. d Immunofluorescence staining of ARP-T1, gamma-tubulin, EHD4, and septin 2 in 48 h of serum-starved hTERT-RPE1 cells. Nuclei are stained with DAPI. Scale bar, 5 um. Higher magnifications of the boxed area are shown on the right three panels. Scale bar, 1 um.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Biological function of BAG2 in gastric cancer cells. (A) Expression of BAG2 in GES1 and several gastric cancer cell lines (MGC803, SGC7901, MKN45, HGC27) showed that BAG2 expressed lowest in GES1 cell line; (B) Interference efficiency of BAG2 -specific siRNAs; (C-G) Effect of BAG2 knockdown on cell viability (C) , colony formation and cell apoptosis (D) , cell proliferation (F) , invasion and migration (G) of gastric cancer cells. ** P < 0.01. 27/NC stands for HGC27/NC and 27/ siBAG2 stands for HGC27/ siBAG2 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Proteomic analysis after knockdown BAG2 . (A) Efficiency verification of shRNA for BAG2 knockdown in the HGC27 cell line (** P < 0.01); (B) Volcanic map of DEPs showed 97 up-regulated and 95 down-regulated (when the difference multiple was more than 1.2 times or
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of BAG2 knockdown on AKT/mTOR pathway, ERK pathway, and EMT process. (A) BAG2 knockdown significantly inhibits the expression of AKT, mTOR, p38, ERK1/2; (B) Immunoprecipitation result showed that BAG2 could bind to ERK1/2; (C) BAG2 knockdown significantly up-regulated E-cadherin and inhibited the expression of N-cadherin, MMP9, Vimentin and Snail, which were related to EMT process. * P < 0.05; ** P < 0.01.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 The expression of BAG2 was regulated by miR186. (A) Prediction of two high score binding sites between miR186 and BAG2 -3'UTR on DIANA website; (B) Overexpression of miR186 inhibits expression of BAG2 protein; (C) Overexpression of miR186 inhibited BAG2 mRNA; (D) The effect of miR186 on the activity of luciferase reporter containing BAG2- 3'UTR-WT , BAG2 -3'UTR-Mut1 , BAG2 -3'UTR-Mut2 or BAG2 -3'UTR-Mut1+2 was tested by luciferase reporter gene (** P < 0.01).