22242-1-AP

antibody from Proteintech Group

Targeting: REST DFNA27, NRSF, XBR

Provider product page for 22242-1-AP

Western blot

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Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [36]
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Validations
Western blot [2]

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Product number: 22242-1-AP - Provider product page
Provider: Proteintech Group
Product name: REST antibody
Antibody type: Polyclonal
Description: KD/KO validated REST antibody (Cat. #22242-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

REST protein structure - 22242-1-AP shown in red.

Submitted references Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis.

Zhu W, Cui Y, Zhou Y, Zheng Y, Huang L, Jin L, Zhang Q, Chen P, Lin M, Ye J, Xiong Y, Huh JY, Hu X, Li X, Luo W, Liang G
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar 25;:e21164

The restoration of REST inhibits reactivity of Down syndrome iPSC-derived astrocytes.

Huang T, Fakurazi S, Cheah PS, Ling KH
Frontiers in molecular neuroscience 2025;18:1552819

The role of REST in regulating the BDNF/TrkB signalling pathway in nano-alumina induced cognitive dysfunction in zebrafish.

Zhang J, Wang S, Jin Z, Ma L, Wang Y, Zhang H, Nie J, Niu Q
Ecotoxicology and environmental safety 2025 Sep 15;303:118998

Glycoprotein 130 improves repressor element‑1 silencing transcription factor‑related axon regenerative capacity in peripheral nerves with aging.

Kawakita S, Naito K, Kubota D, Ueno Y, Negishi-Koga T, Yamamoto Y, Suzuki T, Imazu N, Kawamura K, Hattori N, Ishijima M
Molecular medicine reports 2025 May;31(5)

Small intestinal neuroendocrine tumors lack early genomic drivers, acquire DNA repair defects and harbor hallmarks of low REST expression.

Bolduan F, Müller-Bötticher N, Debnath O, Eichhorn I, Giesecke Y, Wetzel A, Sahay S, Zemojtel T, Jaeger M, Ungethuem U, Roderburg C, Kunze CA, Lehmann A, Horst D, Tacke F, Eils R, Wiedenmann B, Sigal M, Ishaque N
Scientific reports 2025 May 23;15(1):17969

Super-silencer perturbation by EZH2 and REST inhibition leads to large loss of chromatin interactions and reduction in cancer growth.

Zhang Y, Chen K, Tang SC, Cai Y, Nambu A, See YX, Fu C, Raju A, Lebeau B, Ling Z, Chan JJ, Tay Y, Mutwil M, Lakshmanan M, Tucker-Kellogg G, Chng WJ, Tenen DG, Osato M, Tergaonkar V, Fullwood MJ
Nature structural & molecular biology 2025 Jan;32(1):137-149

Dysregulation of REST and its target genes impacts the fate of neural progenitor cells in down syndrome.

Huang T, Fakurazi S, Cheah PS, Ling KH
Scientific reports 2025 Jan 22;15(1):2818

DNMT3b-mediated CpA methylation facilitates REST binding and gene silencing and exacerbates hippocampal demyelination in diabetic mice.

Yao TF, Wang ZY, Sun L, Yu SX, Yu HD, Yang ZZ, Li WZ, Niu L, Sun D, Shi YH, Li JQ, Liu WQ, Liu XZ, Zuo ZF
The Journal of biological chemistry 2025 Feb;301(2):108137

Regulation of nuclear transport of the transcriptional factor REST improves axon regeneration in peripheral nerves.

Suzuki T, Naito K, Kubota D, Ueno Y, Negishi-Koga T, Yamamoto Y, Kawakita S, Imazu N, Kawamura K, Hattori N, Ishijima M
Molecular medicine reports 2025 Dec;32(6)

Silybin ameliorates Pb-induced learning and memory impairment by inhibiting STAT3/hnRNP U/REST and NFκB/hnRNP U.

Zhang W, Chen H, Li Y, Zhu Q, Liu K, Liu X, Wang Y, Zhu G
Environmental toxicology and pharmacology 2025 Dec;120:104859

OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancer.

Liang L, Shen Z, Zhang Y, Cheng Y, Yao B, Feng N, Zhao R
Cell death & disease 2025 Dec 7;17(1):68

EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency.

Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, Iwasaki S, Takahashi K
Science advances 2025 Apr 11;11(15):eadq5484

KIF15 promotes human glioblastoma progression under the synergistic transactivation of REST and P300.

Yu W, Han S, Hu S, Ru L, Hua C, Xue G, Zhang G, Lv K, Ge H, Wang M, Zheng L, Zhou J, Hou S, Teng Y, Deng W, Guo W
International journal of biological sciences 2024;20(13):5127-5144

Functional Analysis of RE1 Silencing Transcription Factor as a Putative Tumor Suppressor in Human Endometrial Cancer.

Abedin Y, Minchella P, Peterson R, Gonnella F, Graham A, Cook I, Javellana M, Jewell A, Spoozak L, Nothnick WB
International journal of molecular sciences 2024 Sep 7;25(17)

EPO modified MSCs protects SH-SY5Y cells against ischemia/hypoxia-induced apoptosis via REST-dependent epigenetic remodeling.

Jiang Y, Li R, Ban Y, Zhang W, Kong N, Tang J, Ma B, Shao Y, Jin R, Sun L, Yue H, Zhang H
Scientific reports 2024 Oct 6;14(1):23252

Prioritization of risk genes in colorectal cancer by integrative analysis of multi-omics data and gene networks.

Zhang M, Wang X, Yang N, Zhu X, Lu Z, Cai Y, Li B, Zhu Y, Li X, Wei Y, Zhang S, Tian J, Miao X
Science China. Life sciences 2024 Jan;67(1):132-148

Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer.

Lu Y, Li H, Zhao P, Tian L, Liu Y, Sun X, Cheng Y
Scientific reports 2024 Feb 5;14(1):2884

Crosstalk between cancer-associated fibroblasts and non-neuroendocrine tumor cells in small cell lung cancer involves in glycolysis and antigen-presenting features.

Lu Y, Li H, Zhao P, Wang X, Shao W, Liu Y, Tian L, Zhong R, Liu H, Cheng Y
Molecular medicine (Cambridge, Mass.) 2024 Dec 25;30(1):274

Targeting of REST with rationally-designed small molecule compounds exhibits synergetic therapeutic potential in human glioblastoma cells.

Panina SB, Schweer JV, Zhang Q, Raina G, Hardtke HA, Kim S, Yang W, Siegel D, Zhang YJ
BMC biology 2024 Apr 12;22(1):83

Epigenetic regulation of GABA catabolism in iPSC-derived neurons: The molecular links between FGF21 and histone methylation.

Ho MF, Zhang C, Moon I, Biernacka J, Coombes B, Ngo Q, Skillon C, Skime M, Oesterle T, Croarkin PE, Karpyak VM, Li H, Weinshilboum RM
Molecular metabolism 2023 Nov;77:101798

Systematic analysis identifies REST as an oncogenic and immunological biomarker in glioma.

Wang G, Yang X, Qi M, Li M, Dong M, Xu R, Zhang C
Scientific reports 2023 Feb 21;13(1):3023

Overexpression of REST Causes Neuronal Injury and Decreases Cofilin Phosphorylation in Mice.

Liu X, Yan J, Liu F, Zhou P, Lv X, Cheng N, Liu L
Journal of Alzheimer's disease : JAD 2022;87(2):873-886

Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through K(v)7.4 channel upsurge in auditory neurons and hair cells.

Zhang H, Li H, Lu M, Wang S, Ma X, Wang F, Liu J, Li X, Yang H, Zhang F, Shen H, Buckley NJ, Gamper N, Yamoah EN, Lv P
eLife 2022 Sep 20;11

Obata H, Naito K, Kikui A, Nakamura S, Suzuki K, Kawakita S, Suzuki T, Goto K, Nagura N, Sugiyama Y, Nagaoka I, Ishijima M
Experimental and therapeutic medicine 2022 Nov;24(5):682

NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice.

Zhang H, Yu S, Xia L, Peng X, Wang S, Yao B
Neurochemical research 2022 Mar;47(3):713-722

Comprehensive Analysis of REST/NRSF Gene in Glioma and Its ceRNA Network Identification.

Zhang Y, Wang Q, Wang Z, Zhang C, Xu X, Xu J, Ren H, Shao X, Zhen X, Zhang L, Yu Y
Frontiers in medicine 2021;8:739624

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Wu Q, Guo J, Liu Y, Zheng Q, Li X, Wu C, Fang D, Chen X, Ma L, Xu P, Xu X, Liao C, Wu M, Shen L, Song H
Science advances 2021 Oct;7(40):eabg1850

Novel REST Truncation Mutations Causing Hereditary Gingival Fibromatosis.

Chen JT, Lin CH, Huang HW, Wang YP, Kao PC, Yang TP, Wang SK
Journal of dental research 2021 Jul;100(8):868-874

Remodeling of the m(6)A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.

Hinger SA, Wei J, Dorn LE, Whitson BA, Janssen PML, He C, Accornero F
Journal of molecular and cellular cardiology 2021 Feb;151:46-55

ALKBH5-mediated m(6)A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling.

He Y, Yue H, Cheng Y, Ding Z, Xu Z, Lv C, Wang Z, Wang J, Yin C, Hao H, Chen C
Cell death & disease 2021 Dec 1;12(12):1121

The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.

Cui D, Dai X, Shu J, Ma Y, Wei D, Xiong X, Zhao Y
Cell death and differentiation 2020 Mar;27(3):1119-1133

Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury.

Bray JK, Elgamal OA, Jiang J, Wright LS, Sutaria DS, Badawi M, Borcyk MG, Liu X, Fredenburg KM, Campbell-Thompson ML, Schmittgen TD
Cell death & disease 2020 Feb 20;11(2):138

Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.

Morris-Blanco KC, Kim T, Bertogliat MJ, Mehta SL, Chokkalla AK, Vemuganti R
Molecular neurobiology 2019 Apr;56(4):2542-2550

Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.

Song Z, Shah SZA, Yang W, Dong H, Yang L, Zhou X, Zhao D
Frontiers in molecular neuroscience 2017;10:128

Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling.

Song Z, Yang W, Zhou X, Yang L, Zhao D
Neuropharmacology 2017 Sep 1;123:332-348

REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling.

Song Z, Zhu T, Zhou X, Barrow P, Yang W, Cui Y, Yang L, Zhao D
Oncotarget 2016 Mar 15;7(11):12035-52

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 22242-1-AP( REST Antibody) at dilution of 1:1500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The REST antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human REST. This antibody recognizes human antigen. The REST antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 22242-1-AP.pdf

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