Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX129144 - Provider product page
- Provider
- GeneTex
- Product name
- REST antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- REST antibody detects REST protein by western blot analysis.A. 30 £gg HeLa whole cell lysate/extract B. 30 £gg HeLa nuclear lysate/extract5 % SDS-PAGEREST antibody (GTX129144) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- U87-MG whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and REST knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and REST knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- REST antibody detects REST protein at cytoplasm and nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. REST antibody (GTX129144) diluted at 1:400.