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- Antibody Data
- Antigen structure
- References [9]
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- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00004849-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004849-M01, RRID:AB_2082318
- Product name
- Mouse Monoclonal CNOT3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CNOT3 - CCR4-NOT transcription complex, subunit 3
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references CNOT3 targets negative cell cycle regulators in non-small cell lung cancer development.
The CCR4-NOT deadenylase complex controls Atg7-dependent cell death and heart function.
Transcriptional Regulator CNOT3 Defines an Aggressive Colorectal Cancer Subtype.
CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.
CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability.
Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.
The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation.
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Shirai YT, Mizutani A, Nishijima S, Horie M, Kikuguchi C, Elisseeva O, Yamamoto T
Oncogene 2019 Apr;38(14):2580-2594
Oncogene 2019 Apr;38(14):2580-2594
The CCR4-NOT deadenylase complex controls Atg7-dependent cell death and heart function.
Yamaguchi T, Suzuki T, Sato T, Takahashi A, Watanabe H, Kadowaki A, Natsui M, Inagaki H, Arakawa S, Nakaoka S, Koizumi Y, Seki S, Adachi S, Fukao A, Fujiwara T, Natsume T, Kimura A, Komatsu M, Shimizu S, Ito H, Suzuki Y, Penninger JM, Yamamoto T, Imai Y, Kuba K
Science signaling 2018 Feb 6;11(516)
Science signaling 2018 Feb 6;11(516)
Transcriptional Regulator CNOT3 Defines an Aggressive Colorectal Cancer Subtype.
Cejas P, Cavazza A, Yandava CN, Moreno V, Horst D, Moreno-Rubio J, Burgos E, Mendiola M, Taing L, Goel A, Feliu J, Shivdasani RA
Cancer research 2017 Feb 1;77(3):766-779
Cancer research 2017 Feb 1;77(3):766-779
CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.
Zheng X, Yang P, Lackford B, Bennett BD, Wang L, Li H, Wang Y, Miao Y, Foley JF, Fargo DC, Jin Y, Williams CJ, Jothi R, Hu G
Stem cell reports 2016 Nov 8;7(5):897-910
Stem cell reports 2016 Nov 8;7(5):897-910
CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability.
Inoue T, Morita M, Hijikata A, Fukuda-Yuzawa Y, Adachi S, Isono K, Ikawa T, Kawamoto H, Koseki H, Natsume T, Fukao T, Ohara O, Yamamoto T, Kurosaki T
The Journal of experimental medicine 2015 Aug 24;212(9):1465-79
The Journal of experimental medicine 2015 Aug 24;212(9):1465-79
Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
Kamon M, Katano M, Hiraki-Kamon K, Hishida T, Nakachi Y, Mizuno Y, Okazaki Y, Suzuki A, Hirasaki M, Ueda A, Nishimoto M, Kato H, Okuda A
Stem cells and development 2014 Sep 15;23(18):2170-9
Stem cells and development 2014 Sep 15;23(18):2170-9
The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.
Doidge R, Mittal S, Aslam A, Winkler GS
PloS one 2012;7(12):e51331
PloS one 2012;7(12):e51331
The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation.
Aslam A, Mittal S, Koch F, Andrau JC, Winkler GS
Molecular biology of the cell 2009 Sep;20(17):3840-50
Molecular biology of the cell 2009 Sep;20(17):3840-50
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Hu G, Kim J, Xu Q, Leng Y, Orkin SH, Elledge SJ
Genes & development 2009 Apr 1;23(7):837-48
Genes & development 2009 Apr 1;23(7):837-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody (M01), clone 4B8.Lane 1: CNOT3 transfected lysate(82 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CNOT3 Antibody (4B8) [H00004849-M01] - CNOT3 monoclonal antibody (M01), clone 4B8 Analysis of CNOT3 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CNOT3 Antibody (4B8) [H00004849-M01] - Detection limit for recombinant GST tagged CNOT3 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CNOT3 Antibody (4B8) [H00004849-M01] - Analysis of monoclonal antibody to CNOT3 on HeLa cell. Antibody concentration 10 ug/ml.
