701159

antibody from Invitrogen Antibodies

Targeting: ZFP42 REX1, ZNF754

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

701159

Provider

Invitrogen Antibodies

Product name

Rex1 Recombinant Rabbit Monoclonal Antibody (14H16L9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Canine

Host

Rabbit

Isotype

IgG

Antibody clone number

14H16L9

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rex1 was performed by loading 20 µg of MDCK whole cell lysate and 5uL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. Rex1 was detected at ~35 kD using a Rex1 recombinant rabbit monoclonal antibody (Product # 701159) at a concentration of 2 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Rabbit IgG-HRP Superclonal™ secondary antibody (Product # A27036) at a concentration of 1 µg/mL for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product # 34080).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rex1 in A431 whole cell extracts using a Rex1 recombinant rabbit monoclonal antibody (Product # 701159) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~35kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Rex1 in HeLa cells using a Rex1 recombinant rabbit monoclonal antibody (Product # 701159) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Rex1.