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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 710190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Rex1 Recombinant Polyclonal Antibody (14HCLC)
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 14HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division.
Junyent S, Reeves JC, Szczerkowski J, Garcin CL, Trieu TJ, Wilson M, Lundie-Brown J, Habib SJ
eLife 2021 May 24;10
eLife 2021 May 24;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rex1 was performed by loading 20 µg of HeLa, 3T3, NRK, MDCK, and Cos7 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. Rex1 was detected at ~35 kD using a Rex1 Recombinant Rabbit Polyclonal Antibody (Product # 710190) at a concentration of 2 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Rabbit IgG-HRP Superclonal secondary antibody (Product # A27036) at a concentration of 1 µg/mL for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product # 34080).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rex1 in whole cell extracts of A431 (lane 1) and rat kidney lysate (lane 2) using a Rex1 Recombinant Rabbit Polyclonal Antibody (Product # 710190) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~35kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Rex1 in HeLa cells using a Rex1 Recombinant Rabbit Polyclonal Antibody (Product # 710190) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Rex1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of Rex1 performed using cross-linked chromatin from 1 x 10^6 HCT116 human colon carcinoma cells treated with serum for 0, 15, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a Rex1 Recombinant Rabbit Polyclonal Antibody (Product # 710190). Chromatin aliquots from ~1 x 10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the human Egr-1 locus, or exon-1 or exon-2 of Egr-1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.
