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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-54648 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KGSETDSAQD QPVKMNSLPA ERIQEIQKAI ELFSVGQGPA KTMEEASKRS YQFWDTQPVP KLGEVVNTH Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions.
Govatati S, Pichavaram P, Janjanam J, Guo L, Virmani R, Rao GN
Arteriosclerosis, thrombosis, and vascular biology 2020 May;40(5):1256-1274
Arteriosclerosis, thrombosis, and vascular biology 2020 May;40(5):1256-1274
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NMT1 in human cell line HDLM-2 using a NMT1 Polyclonal Antibody (Product # PA5-54648).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NMT1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a NMT1 Polyclonal Antibody (Product # PA5-54648).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NMT1 in human cell line U-2 OS shows positivity in cytoplasm & actin filaments. Samples were probed using a NMT1 Polyclonal Antibody (Product # PA5-54648).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NMT1 in human placenta using a NMT1 Polyclonal Antibody (Product # PA5-54648) shows cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NMT1 in human cerebellum using NMT1 Polyclonal Antibody (Product # PA5-54648) shows moderate to strong cytoplasmic positivity in Purkinje cells and basket cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NMT1 in human liver using NMT1 Polyclonal Antibody (Product # PA5-54648) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NMT1 in human skeletal muscle using NMT1 Polyclonal Antibody (Product # PA5-54648) shows no cytoplasmic positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NMT1 in human cerebral cortex using NMT1 Polyclonal Antibody (Product # PA5-54648) shows moderate to strong cytoplasmic positivity in neuronal cells.
