PA5-78082

antibody from Invitrogen Antibodies

Targeting: NMT1 NMT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78082

Provider

Invitrogen Antibodies

Product name

NMT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, HeLa, HepG2

Concentration

0.42 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NMT1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with NMT1 polyclonal antibody (Product # PA5-78082) using a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NMT1 Polyclonal Antibody (Product # PA5-78082). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 Polyclonal Antibody (Product # PA5-78082) diluted at 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NMT1 Polyclonal Antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMT1 protein stained by NMT1 Polyclonal Antibody (Product # PA5-78082) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NMT1 Polyclonal Antibody detects NMT1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NMT1 Polyclonal Antibody (Product # PA5-78082) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NMT1 Polyclonal Antibody detects NMT1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NMT1 Polyclonal Antibody (Product # PA5-78082) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NMT1 Polyclonal Antibody detects NMT1 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. NMT1 Polyclonal Antibody (Product # PA5-78082) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.