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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31368 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STRN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (92%), Xenopus laevis (94%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer.
Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling.
Tang Y, Fang G, Guo F, Zhang H, Chen X, An L, Chen M, Zhou L, Wang W, Ye T, Zhou L, Nie P, Yu H, Lin M, Zhao Y, Lin X, Yuan Z, Jiao S, Zhou Z
Cancer cell 2020 Jul 13;38(1):115-128.e9
Cancer cell 2020 Jul 13;38(1):115-128.e9
Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling.
Tang Y, Chen M, Zhou L, Ma J, Li Y, Zhang H, Shi Z, Xu Q, Zhang X, Gao Z, Zhao Y, Cheng Y, Jiao S, Zhou Z
Cell discovery 2019;5:3
Cell discovery 2019;5:3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using STRN3 Polyclonal Antibody (Product # PA5-31368). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 7.5% SDS PAGE. STRN3 Polyclonal Antibody (Product # PA5-31368) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of STRN3 in methanol-fixed MCF-7 cells using a STRN3 polyclonal antibody (Product # PA5-31368) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using STRN3 (Product # PA5-31368) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 10 Dynamic assembly of STRIPAK complexes. a Co-immunoprecipitation and immunoblot analysis of the endogenous interactions between core STRIPAK components and STRN3 in HEK293FT cells at two different cell densities. b Co-immunoprecipitation and immunoblot analysis of the endogenous interactions between core STRIPAK components and MST1 in HEK293FT cells at two different cell densities. c Confocal microscope analysis of the colocalization of MST2 or STRIP1 with STRN3 in HEK293FT cells at two different cell densities. The rate of colocalization was quantified and shown (right). d Model of the dynamic assembly of the STRIPAK complex induced by different cell densities
