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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A300-843A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIP4/ASC-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4-ASC-1 antibody (Product # A300-843A lot 4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (Product # A300-843A lot 4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRIP4/ASC-1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP4/ASC-1 antibody A300-843A (lot A300-843A-3) used for IP at 3 µg per reaction. TRIP4/ASC-1 was also immunoprecipitated by rabbit anti-TRIP4/ASC-1 antibody A300-203A. For blotting immunoprecipitated TRIP4/ASC-1, A300-843A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC-1 antibody A300-843A (lot A300-843A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRIP4/ASC-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TRIP4/ASC-1 (Cat. No. A300-843A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TRIP4/ASC-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (Product # A300-843A; Lot 4) used for IP at 6 µg per reaction. TRIP4/ASC-1 was also immunoprecipitated by a previous lot of this antibody (Product # A300-843A; Lot 3) and a second antibody against a different epitope of TRIP4/ASC-1 (Product # A300-203A). For blotting immunoprecipitated TRIP4/ASC-1, Product # A300-843A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
