PA5-96296

antibody from Invitrogen Antibodies

Targeting: MYCBP AMY-1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96296

Provider

Invitrogen Antibodies

Product name

MYCBP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAHYKAADSK REQFRRYLEK SGVLDTLTKV LVALYEEPEK PNSALDFLKH HLGAATPENP EIELLRLELA EMKEKYEAIV EENKKLKAKL AQYEPPQEEK RAE; Positive Samples: A-549, K-562; Cellular Location: Cytoplasm, Mitochondrion, Nucleus

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.95 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MYCBP in extracts of various cell lines. Samples were incubated with MYCBP Polyclonal antibody (Product # PA5-96296) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYCBP in paraffin-embedded human thyroid cancer. Samples were incubated with MYCBP Polyclonal antibody (Product # PA5-96296) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYCBP in paraffin-embedded human placenta. Samples were incubated with MYCBP Polyclonal antibody (Product # PA5-96296) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYCBP in paraffin-embedded human placenta. Samples were incubated with MYCBP Polyclonal antibody (Product # PA5-96296) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.