Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MA5-14956 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUFU Monoclonal Antibody (C.60.7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- C.60.7
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KD of SUFU was achieved by transfecting HeLa with SUFU specific siRNAs (Silencer® select Product # s268522, s529326). Western blot analysis (Fig. a) was performed using whole cell extracts from the SUFU KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with SUFU Monoclonal Antibody (C.60.7) (Product # MA5-14956, 1:1000 dilution) and Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SUFU.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SUFU Monoclonal Antibody (C.60.7) (Product # MA5-14956) and a 54 kDa band corresponding to SUFU was observed in all the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of DU 145 (Lane 1), SH-SY5Y (Lane 2), PANC-1 (Lane 3), U-2 OS (Lane 4), A-431 (Lane 5), and HeLa (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).