Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-29952 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUFU Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SUFU Polyclonal Antibody (Product # PA5-29952). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. Suppressor of Fused antibody . SUFU Polyclonal Antibody (Product # PA5-29952) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SUFU Polyclonal Antibody (Product # PA5-29952) and a 43kDa band corresponding to SUFU was observed in Mouse Adult Kidney and Mouse Adult Brain, and not in Mouse Pup Kidney and Mouse Post-natal (Day1) Brain, which are reported negative for SUFU expression. Tissue extracts (30 µg lysate) of Mouse Pup Kidney (Lane 1), Mouse adult Kidney (Lane 2), Mouse Post-natal (Day1) Brain (Lane 3) and Mouse adult Brain (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Suppressor of Fused in paraformaldehyde-fixed HeLa cells using a Suppressor of Fused polyclonal antibody (Product # PA5-29952) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Suppressor of Fused (Product # PA5-29952) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Suppressor of Fused antibody [C1C4] detects Suppressor of Fused protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Suppressor of Fused antibody [C1C4] (Product # PA5-29952) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Suppressor of Fused antibody [C1C4] detects Suppressor of Fused protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Suppressor of Fused antibody [C1C4] (Product # PA5-29952) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.