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LearnSTJ27419
antibody from St John's Laboratory
Targeting: HIF1AN
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- STJ27419 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-HIF1AN antibody (1-349) (STJ27419)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-HIF1AN/FIH1 (1-349) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store in a freezer at-20°C and avoid freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using HIF1AN/FIH1 antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and HIF1AN/FIH1 rabbit polyclonal antibody knockout (KO) 293T cells, using HIF1AN/FIH1 rabbit polyclonal antibody antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of L929 cells using HIF1AN/FIH1 antibody (STJ27419) at dilution of 1:100. Blue: DAPI for nuclear staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HIF1AN/FIH1 antibody (STJ27419). Western blot was performed from the immunoprecipitate using HIF1AN/FIH1 antibody (STJ27419) at a dilition of 1:1000.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
